Bio – 219 Questions With Complete Solutions
How long is the lux operon 8.5 kb long
A. fischeri Bacteria found throughout marine environments
Auto-induced bioluminescence An operon that allows A. fischeri to glow under the right
conditions
Vector types Plasmids
Phagemids
Bacteriophage Vect...
A. fischeri Bacteria found throughout marine environments
Auto-induced bioluminescence An operon that allows A. fischeri to glow under the right
conditions
Vector types Plasmids
Phagemids
Bacteriophage Vectors
Cosmid Vectors
Bacteria/Yeast artificial chromosomes
Phagemids phage + plasmid
-package ssDNA in presence of a helper phage
Bacteriophage Vectors Based on the lambda virus
,Bio – 219 Questions With Complete Solutions
larger capacity >25 kb per virus
Cosmid Vectors lambda cos sites + plasmid
->35 kb (35-45kb) inserts availabe
-can be packed in viral proteins for infectivity
-low copy number in bacteria
Bacterial/yeast artificial chromosomes -large, low copy number 1-2 per cell
->300 kb inserts are acceptable
-Good for sequencing
We chose phagemids because it will operate as a plasmid
its small circular DNA
-1-200kb
-can accept DNa fragments from 1-10kb
-extra-chromosomal
, Bio – 219 Questions With Complete Solutions
Self replicating
Exist in nature to carry antibiotic resistance and other survival genes
can be transmitted between two bacteria
been engineered into excellent vectors
What makes a good plasmid vector? Size
-large enough to hold workable quantities of foreign DNA
-Small enough to be retained by the host
-small enough to be able to distinguish from host chDNA
High copy number
-50 to 100 per cell
Origin of replication (ORI)
- Must be recognizable by the host's replication machinery
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