Bio 219 Final Questions With Complete
Solutions
There are two purposes to our quarter-long project. What are they? a. The first aspect is to
isolate the lux operon from Aliivibrio fischeri genomic DNA and transform E. coli to induce
bioluminescence
b. The second goal is to quantify gene exp...
Molecular oxygen oxidizes flavin mononucleotide and a long chain aliphatic aldehyde to an
aliphatic carboxylic acid. Forms an excited hydroxyfavin intermediate which is dehydrates to
product of FMN to emit light
, Bio 219 Final Questions With Complete
Solutions
What is quorum sensing? Regulation of gene expression in response to changes in cell-
population density
Describe auto-induction Bacteria release a small metabolic product (N-acyl homoserine-
lactone). AHL diffuses freely across cell membrane into an extracellular environment. Density
goes up, more inducer is increased. When a critical concentration of the inducer is present, the
inducer diffuses back into the cells and interacts with specific factors which results in gene
expression.
What is the E.Coli LuxR homolog? Can E-Coli use quorum sensing? If so how does E-Coli do
this in nature? E.coli encodes the LuxR homolog, SdiA to detect AHL. It lacks the gene to
make the autoinducer but can response to AHL. The SdiA detects AHL and induces up-regulation
of gadAx operon which contains the GadA gene.
a. What is the GADAX operon? What does it encode? What gene are we focusing on? What
should happen to that gene's expression in the presence of the Lux operon? What reference gene
will we be using as a comparison to the gene in question? GadA encodes for a glutamate
decarboxylase that plays a role in acid tolerance. If lux is present, then the gadA gene is
significantly upregulated due to AHL being present.
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