Why is desiccating your inoculating loop important? When should this be done? -
️️To ensure it is sterilized, done so no cross contamination happens
Bacteria in the Mycobacterium genus have the presence of what component in the cell
wall that causes them to be acid-fast? - ️️Peptidoglyca...
Microbio 150 Lab exam 1 PVCC
Why is desiccating your inoculating loop important? When should this be done? -
✔️✔️To ensure it is sterilized, done so no cross contamination happens
Bacteria in the Mycobacterium genus have the presence of what component in the cell
wall that causes them to be acid-fast? - ✔️✔️Peptidoglycan
Where should used gloves be disposed of in the microbiology lab - ✔️✔️In the bio-
hazard waste container
Before putting a dust cover over the microscope and placing it in the cabinet, which
objective lens should be rotated over the stage - ✔️✔️4x
On the microscope, what concentrates the light on the specimen? - ✔️✔️Condenser
lens
On the microscope, what bends light passing through the specimen to create a real
image - ✔️✔️Objective lens
On the microscope, what allows for more or less light to be directed onto the specimen -
✔️✔️Iris diaphragm
When preparing to view a specimen on a compound microscope in the lab, one should
__________ begin with the 4x objective - ✔️✔️Always
On the microscope, what gives you clarity of the image - ✔️✔️Resolution
During an aseptic transfer, when do you pass the mouth of the test tube through the
flame? - ✔️✔️Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain a pure
culture from a __________. - ✔️✔️Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to - ✔️✔️Attach it
firmly to the slide
The term that refers to the purposeful addition of microorganisms into a nutrient medium
is - ✔️✔️Inoculation
, True or false: In the microbiology lab, it is acceptable to move your loop around while
waiting for it to dry. - ✔️✔️False
Which stain was used during the negative staining procedure? - ✔️✔️Nigrosin
Which stain was used during the simple staining procedure? - ✔️✔️Methylene blue
True or false: During a negative stain, the bacteria are heat fixed. - ✔️✔️False
After the decolorizer has been added, gram-positive organisms appear __________
and gram-negative organisms appear __________. - ✔️✔️Purple; colorless
Why is iodine (or a mordant) used in the Gram staining process? - ✔️✔️It forms a
complex with crystal violet
True or false: bacterial slides are not heat-fixed prior to beginning to Gram staining. -
✔️✔️False
When completing a bacterial transfer from a broth, where should the cap be located
while you are inserting your inoculating instrument in the bacterial growth? - ✔️✔️The
cap should be held in the pinky of your dominant hand
- ✔️✔️Agar slant
- ✔️✔️Broth tube
- ✔️✔️Agar plate
solidified in an upright angle - ✔️✔️Agar deep
What is the purpose of a simple stain? - ✔️✔️To quickly visualize microorganisms
because they are virtually transparent.
True or false: Basic stains work because the positively charged chromophore repels the
positively charged bacterial cell. - ✔️✔️False
What is the colored, charged region of a stain called? - ✔️✔️The chromophore
- ✔️✔️Negative stain
Positive or negative - ✔️✔️Negative gram stain
Positive or negative - ✔️✔️Positive gram stain
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