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gel electrophoresis quizzes with correct answers What is the purpose of gel electrophoresis? - ANSWER-To separate DNA fragments based on their size using electricity What does "phoresis" mean? - ANSWER-To transmit (move location, migrate) What three gel ingredients are required for gel electro...

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  • November 12, 2024
  • 6
  • 2024/2025
  • Exam (elaborations)
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EmilyCharlene
gel electrophoresis quizzes with
correct answers

What is the purpose of gel electrophoresis? - ANSWER✔✔-To separate DNA fragments

based on their size using electricity


What does "phoresis" mean? - ANSWER✔✔-To transmit (move location, migrate)


What three gel ingredients are required for gel electrophoresis? - ANSWER✔✔-

Agarose, buffer, and distilled H2O


What is agarose? - ANSWER✔✔-A sugar found in seaweed


What is a characteristic of agarose? - ANSWER✔✔-It is gelatin-like


What purpose does agarose serve in gel electrophoresis? - ANSWER✔✔-It is the matrix

(obstacle course) for DNA fragments (it separates them by size)


What is the buffer? - ANSWER✔✔-A salt solution


What purpose does the buffer serve in gel electrophoresis? - ANSWER✔✔-It maintains

pH (at 7 which is neutral) and conducts electricity which allows the DNA fragments to

migrate through the agarose gel




Copyright ©EMILYCHARLENE2025. ALL RIGHTS RESERVED
Page 1/6

, What purpose does the distilled H2O serve in gel electrophoresis? - ANSWER✔✔-It is

the solvent


Why does the H2O need to be distilled? - ANSWER✔✔-It contains no chlorine or other

contaminants that could damage DNA


How is the agarose power measured? - ANSWER✔✔-On an electronic balance, after

zeroing out the weighing tray


How is the distilled H2O measured? - ANSWER✔✔-In a graduated cylinder (measured

to the meniscus)


What equipment is required for gel electrophoresis? - ANSWER✔✔-Casting tray (baby

gates, combs), chambers, lids, power sources


What is the process of gel electrophoresis? - ANSWER✔✔-The DNA is loaded into wells

formed by combs in the agarose gel, buffer is added, then an electric current is applied

and the DNA migrates to the bottom


How are the DNA fragments filtered in the agarose gel during gel electrophoresis? -

ANSWER✔✔-The largest fragments get stuck at the top, while the smallest fragments

move towards the bottom of the gel


Why does DNA migrate? - ANSWER✔✔-The phosphate groups on DNA have a

negative charge and migrate towards the positive electrode (red) because opposites

attract

Copyright ©EMILYCHARLENE2025. ALL RIGHTS RESERVED
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