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Building a phenotype-o-mat: A low-cost DIY plate reader for high-throughput phenotype measurements
Ben Braverman1
1Arcadia Science
Ben Braverman: This device was made by biologists, not electrical engineers. If anything is unclear
please let us know!
MAR 15, 2024 Arcadia Science
Arcadia Science
Arcadia Science
DOI:
dx.doi.org/10.17504/protocols.io.y
xmvm3r3ol3p/v1
Protocol Citation: Ben
Braverman 2024. Building a
phenotype-o-mat: A low-cost DIY
plate reader for high-throughput
phenotype measurements.
protocols.io
https://dx.doi.org/10.17504/protoc
ols.io.yxmvm3r3ol3p/v1
License: This is an open
access protocol distributed under
the terms of the Creative
Commons Attribution License,
which permits unrestricted use,
distribution, and reproduction in
any medium, provided the original
author and source are credited
Protocol status: Working
We use this protocol and it's
working
protocols.io |
Oct 15 2024 1
https://dx.doi.org/10.17504/protocols.io.yxmvm3r3ol3p/v1
,Created: Feb 13, 2024 ABSTRACT
Last Modified: Mar 15, 2024
PROTOCOL integer ID: 95184
Keywords: diy, phenotype,
measurement, plate reader,
imaging, imager, phenotype-o-
mat, low-cost, cheap, assemble,
assembly, high-throughput, high
throughput, cell biology, cell bio,
organism, 96-well, single-cell,
bulk
The phenotype-o-mat with labeled parts and approximate costs
We've developed a DIY, low-cost, 96-well plate imager called the phenotype-o-mat. This
protocol outlines the steps to assemble it yourself. We cover:
1) 3D-printing components
2) Adding threaded inserts
3) Soldering LEDs
4) Setting up the Arduino Nano Every
5) Connecting the BlackFly camera
6) Running an assay
protocols.io |
Oct 15 2024 2
https://dx.doi.org/10.17504/protocols.io.yxmvm3r3ol3p/v1
, While you can 3D-print a few of the components, the rest are commercially available.
Check out the "Materials" tab to see what you'll need before beginning assembly. The
necessary 3D-printing files and code are available in our phenotype-o-mat GitHub repo.
If you have any questions or suggestions about assembly, please feel free to reach out at
ben.braverman@arcadiascience.com or comment on our forthcoming phenotype-o-mat
pub at research.arcadiascience.com.
IMAGE ATTRIBUTION
Arcadia Science
protocols.io |
Oct 15 2024 3
https://dx.doi.org/10.17504/protocols.io.yxmvm3r3ol3p/v1
, MATERIALS
LEDs
Three white LED backlight modules (Large, 45 mm × 86 mm)
Five super bright blue 5 mm LEDs (25 pack)
Five super bright red 5 mm LEDs (25 pack)
Five super bright yellow 5 mm LEDs (25 pack)
Five super bright green 5 mm LEDs (25 pack)
Camera
BFS-U3-16S2M-CS USB camera
12 mm CS mount lens (The one we've linked is part of a kit)
USB-A 3.0 "male" to USB micro B "male" cable (The screw-locking and down-angle
version helps keep the connection secure and removes some tension as the cable pulls
on the camera. We recommend at least 3 m in length to accommodate different
physical setups)
25 mm optical filters (optional)
Hardware
Black PLA filament
Clear PLA filament
T-slotted framing rail (single four-slot rail, black, 20 mm square, solid - 40 cm)
Assorted threaded inserts
M3 thumb screws
Custom LED ring PCB
18 100-ohm resistors
Five 150-ohm resistors
Solder
Copper wire
Colored wire (black, white, green, blue, yellow, and red)
Arduino Nano Every
Heat shrink
Black corrugated plastic (at least 24 x 32 in), 4 mm thick
Black duct tape
Lab tape (optional)
Zip ties
Rubber bands (optional)
Tools
FDM 3D printer
Grippers
protocols.io |
Oct 15 2024 4
https://dx.doi.org/10.17504/protocols.io.yxmvm3r3ol3p/v1
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