(MB) ASCP MOLECULAR BIOLOGY
EXAM LATEST 2025 ACTUAL EXAM
QUESTIONS AND DETAILED CORRECT
ANSWERS (VERIFIED ANSWERS) | A+
GRADE
A patient with impaired judgment, personality changes,
signs of abnormal body movements and depression
comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the
cause of those clinical manifestations. A blood specimen
was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard
PCR indicates that patient has Huntington Disease, HD.
Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene Correct Answer
85 CAG repeats in the Huntington gene
Which two HPV types are responsible for most cases of
cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 Correct Answer 16 and 18
,Replication forks, known as origins of DNA replication, are
created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase Correct Answer Helicase
Mutation in what gene is associated with Fragile X
syndrome? Correct Answer FMR1
Mantle cell lymphoma (MCL) is caused by what
translocation? Correct Answer t(11;14)
This polymerase is involved in "initiation of DNA replication
and has primase activity": Correct Answer Pol α
Its discovery shed light on why there is simultaneous,
though not continuous, synthesis of DNA on both leading
and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments Correct Answer Okazaki fragments
What gene is measured following treatment with imatinib
(Gleevec)?
FLT3
BCR/Abl
Jak2
MAPK Correct Answer BCR/Abl
,What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second Correct Answer 50 nucleotides
per second
This polymerase is involved in "replicates mitochondrial
DNA": Correct Answer Pol γ
A patient with impaired judgment, personality changes,
signs of abnormal body movements and depression
comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the
cause of those clinical manifestations. A blood specimen
was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods
would best accomplish this task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR Correct Answer Standard PCR
Which of the following storage options is optimal for
storing isolated DNA for a period greater than seven
years?
22-25ºC
2-8ºC
-20ºC
-70ºC Correct Answer -70ºC
, Consider a hypothetical mutation involving gene X. Let's
say you amplify a specific exon, say exon 11, of that gene
then you cut it with restriction enzyme W. In a person
without the mutation, cutting the gene with restriction
enzyme W generates two fragments of sizes, 100 bp and
250 bp. Suppose a C>T mutation in gene X deletes a
restriction site, yielding a fragment of 350 bp. You would
expect a heterozygous person for gene X to have these
fragments on a restriction gel:
+/+ = 350 bp; 250 bp; 100 bp;
m/+ = Only the 350 bp
m/+ = 350 bp; 250 bp; 100 bp
m/m = 350 bp; 250 bp Correct Answer m/+ = 350 bp; 250
bp; 100 bp
Which of the following will more likely lower stringency
conditions in the washing step of a hybridization
experiment?
Increase the concentration of salt in the wash solution
buffer
Increase the temperature from, say 68°C to 75°C
Use a probe with a higher density of GC base pairs as
compared to one with a lower GC base pair density
Remove formamide from the wash solution buffer Correct
Answer Increase the concentration of salt in the wash
solution buffer
What enzyme is involved in LCR? Correct Answer DNA
Ligase
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