CHM-365 Quiz 3 UPDATED ACTUAL Questions and CORRECT Answers
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Course
CHM-365
Institution
CHM-365
CHM-365 Quiz 3 UPDATED ACTUAL
Questions and CORRECT Answers
adjusted retention time - CORRECT ANSWER- In chromatography, this parameter is tr'
= tr - tm, where tr is the retention time of a solute and tm is the time needed for mobile phase
to travel the length of the column.
adsorption chro...
CHM-365 Quiz 3 UPDATED ACTUAL
Questions and CORRECT Answers
adjusted retention time - CORRECT ANSWER- ✔✔In chromatography, this parameter is tr'
= tr - tm, where tr is the retention time of a solute and tm is the time needed for mobile phase
to travel the length of the column.
adsorption chromatography - CORRECT ANSWER- ✔✔a technique in which solute
equilibrates between the mobile phase and adsorption sites on the stationary phase
This has a solid stationary phase and a liquid or gaseous mobile phase
affinity chromatography - CORRECT ANSWER- ✔✔A technique in which a particular
solute is retained by a column by virtue of a specific interaction with a molecule covalently
bound to the stationary phase.
asymmetry factor (B/A) - CORRECT ANSWER- ✔✔In chromatography, the parameter
describing the shape of a peak. B is the distance from the peak apex to the back of the peak
measured at 10% of peak height. A is the distance from the peak apex to the front of the peak
at 10% of peak height. B/A 5 1 is a symmetrical peak. B/A . 1 is tailing and B/A , 1 is fronting
baseline resolution - CORRECT ANSWER- ✔✔Occurs in chromatography when two
adjacent peaks are sufficiently resolved that the signal between the peaks returns to baseline
(resolution > 1.5). Allows determination of accurate peak areas.
Chromatagram - CORRECT ANSWER- ✔✔A graph showing chromatography detector
response as a function of elution time or volume.
Diffusion - CORRECT ANSWER- ✔✔Net transport of a solute from a region of high
concentration to a region of low concentration caused by the random movement of molecules
in a liquid or gas (or, very slowly, in a solid).
diffusion coefficient (D) - CORRECT ANSWER- ✔✔Defined by Fick's first law of diffusion
J = -D(dc/dx), where J is the rate at which molecules diffuse across a plane of unit area and
dc/dx is the concentration gradient in the direction of diffusion.
, distribution coefficient (D) - CORRECT ANSWER- ✔✔For a solute partitioned between two
phases, the ____________ ___________ is the total concentration of all forms of solute in
phase 2 divided by the total concentration in phase 1.
Eluate - CORRECT ANSWER- ✔✔What comes out of a chromatography column. Also
called effluent.
eluent - CORRECT ANSWER- ✔✔Solvent applied to the beginning of a chromatography
column.
Elution - CORRECT ANSWER- ✔✔Process of passing a liquid or a gas through a
chromatography column.
extraction - CORRECT ANSWER- ✔✔Process in which a solute is transferred from one
phase to another. Analyte is sometimes removed from a sample by __________ into a solvent
that dissolves the analyte.
gel filtration chromatography - CORRECT ANSWER- ✔✔a type of column chromatography
that separates proteins based on their size using size-exclusion beads; also called size-
exclusion chromatography
gel permeation chromatography - CORRECT ANSWER- ✔✔also known as size exclusion,
molecular exclusion, and molecular sieve chromatography; a form of chromatography that
utilizes a gel stationary phase to separate molecules according to their molecular size in
solution
Nonpolar Organic Solvents (mobile phase)
Hydrophobic beads (stationary phase)
ion exchange chromatography - CORRECT ANSWER- ✔✔A technique in which solute ions
are retained by oppositely charged sites in the stationary phase.
laminar flow - CORRECT ANSWER- ✔✔Motion with a parabolic velocity profile of fluid
through tube. Motion is fastest at the center and zero at the walls.
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