PORTAGE LEARNING MICRO FINAL EXAM
Questions and answers Latest Versions
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PRE-Exam
1. True or False: A virus is considered a microorganism.
False. Viruses are not living and as such are not considered microorganisms. Viruses can, however, be classified as
microbes, a more general term that includes microorganisms and viruses.
2. What is the smallest biological unit of life?
A cell.
3. At a generalized level, all cells are comprised of what?
Macromolecules*
*A student may also answer: Proteins, Lipids, Nucleic acids and Polysaccharides but they must answer with all
four to be fully correct.
1. Which of the following microorganisms are considered to be Eukarya? Select all that apply.
A. Animalia
B. Plantae
C. Fungi
D. Protista
A,B,C,D
2. True of False: All multicellular microorganisms classified as Animalia are heterotrophic.
True
3. Microorganisms classified as Plantae obtain most of their energy by converting energy into
energy.
Light (sunlight); chemical (sugars)
1. Define catabolism.
Catabolism is the process of breaking down larger molecules into useful energy sources.
2. Upon cellular injury, which metabolic process is involved during the growth and repair phases of the cell?
The anabolic process would be active as it (by definition) is involved in the building up of small complexes into
larger complexes.
1. In phosphorylation, the light reactions always occur where?
The process of converting light energy into chemical energy (photophosphorylation) always occurs in the
membrane.
2. True or False: The Calvin cycle must occur in the absence of light.
False. The term ‘dark reactions’ (also known as the Calvin Cycle) simply denotes the second stage in
photosynthesis—dark reactions do not actually require darkness in order to occur.
1. Assuming a constant (non-adjustable) light source power, identify the part of the microscope you would
adjust to limit the amount of light entering the microscope.
Select all that apply.
A. Objective
B. Condenser
C. Iris diaphragm
,D. Eye piece
1
C. The iris controls the amount of light that passes through the sample and into the objective lens. Thus, it can
be adjusted (opened or closed) to alter the amount of light.
2. What is the total magnification (relative to your eye) of a sample imaged with a 60x objective and a 10x
eyepiece? Show your math.
60 x 10 = 600x magnification
2
, 1. Identify what type of electron microscope was used to capture the following image and explain your
choice.
The image was captured using a Scanning Electron Microscope (SEM). The above image shows the trademark
‘shell’ image (no subcellular organelles are visible) reminiscent of SEM. Only TEM is capable of visualizing
subcellular substrucutres.
1. True or False: LB agar is classified as a selective, non-differential media.
False. LB agar is the most basic type of agar and like LB media supports the growth of virtually all
microbes without restriction.
2. What is agar used for in microbiology?
Agar is used to create a solid, smooth surface on which microbes can grow.
1. True or False. When performing a dilution streak a new (or sterilized) loop is not required for each phase
as long as the bacterial culture is pure.
False.
2. The number of phases (3 vs. 4) and/or the number of times a loop passes through a previous phase (once
vs. multiple times) is acceptable provided what happens?
Either deviation is acceptable in practice provided the resulting gradient contains within it the growth
of individual colonies—if not, the experiment must be repeated.
3. True or False. Pathogenic strains of bacteria tend to grow slower than normal non-pathogenic bacterial
strains.
False.
1. Match the following symptoms with their respective diseases:
1. Folliculitis A. Childhood skin disease near mouth/
D nose
2. Scalded-skin
B. Infection of thin, transparent scleral
syndrome
tissue
E
3. Impetigo
C. Infection occurs at time of birth
A
4. Conjunctivitis
D. Pus-filled lesions on skin or hair
B
3
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