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EEOB 3520 Exam 1 Questions and Answers (100% Pass)

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Difference between histology vs microanatomy histology is the study of plant and animal tissues while microscopic anatomy is the analysis of cells, tissues, organs, and organ systems What does fixation do? Fixation terminates metabolism, stops enzymes, destroys pathogen, ( fresh brain is...

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  • November 18, 2024
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EEOB 3520 Exam 1 Questions and
Answers (100% Pass)
Difference between histology vs microanatomy


✓ histology is the study of plant and animal tissues

✓ while microscopic anatomy is the analysis of cells, tissues, organs, and

organ systems




What does fixation do?


✓ Fixation terminates metabolism, stops enzymes, destroys pathogen, (

fresh brain is very soft, liquidy, fixation increases rigidity




What is formaldehyde?


✓ , formaldehyde is a gas, you don't preserve things in formaldehyde, you

have to put it in water, becomes formalin




What does formalin do


✓ formalin cross links proteins- forms bridges and prevents them from

being degraded




What happens after fixation?


Master01 |Excellent Study Material| Latest update

, 1|Page | © copyright Master01| Grade A+

✓ tissue needs to be dehydrated, clear it and prepare to be embedded

in medium to be sliced

✓ Dehydration is necessary because tissue is 60% water, so need to

remove water




What is clearing


✓ run sample through ↑ concentrations of alcohol then

✓ infiltrate with solvent appropriate for embedding medium

✓ - Clearing means you go from alcohol to something that is miscible in

both alcohol and wax, xylene or toluene( petroleum products that can

dissolve wax)




How does embedding work?


✓ Material for embedding is wax, paraffin. Paraffin is not easy to remove

not water or alcohol soluble




Processing of tissue


✓ - machine goes up and down and agitates cassetes in bins of liquids

and rotates and drops down in a new bin

✓ Then you end up with a piece of tissue in wax




Master01 |Excellent Study Material| Latest update

, 1|Page | © copyright Master01| Grade A+

How do you embed tissue?


✓ infiltrate tissue block with embedding medium

✓ place in block, fill with liquid medium, and let solidify

✓ There are molds that you put tissue in block in mold filled with melted

wax




How do you section and mount tissue?


✓ use a microtome

✓ 5-15 μm thick

✓ individual or serial sections

✓ There is a blade and a block, evry time you turn handle the block

advances, so little slivers of block gets cut. They are so thin, they stick to

each other




After mounting you...




Master01 |Excellent Study Material| Latest update

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