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UNE Biochem Midterm Exam with correct Answers
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UNE Biochem Midterm Exam with correct Answers
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UNE Biochem Midterm Exam withcorrect Answers
Changes in the physiological variables listed below can alter the affinity of hemoglobin
for oxygen. Which of the following will lower the affinity of hemoglobin for oxygen? -
Answers -increase 2,3 biphosphoglycerate (BPG)
Allosteric activators of hemoglobin will increase substrate binding through which of the
following mechanisms? - Answers -Binding the protein, and keeping it in the "R"
conformation
Which of the following is the best description of primary protein structure? - Answers -A
newly synthesized strand of amino acids
Movement of ammonia from an amino acid to an α-keto acid involves a family of
enzymes best categorized as which of the following? - Answers -transferases
A 19-year-old boy is diagnosed with Creutzfeldt-Jakob Disease which is caused by the
introduction of an amyloid fold in the disease-causing protein. The introduction of this
fold causes the protein to transition from a primarily α-helical structure to an aggregate
of mostly β-sheets. This change in protein structure (leading to disease) is best
attributed to changes in which of the following? - Answers -folding of the secondary
structures
Which of the following best describes tertiary structure? - Answers -Interactions
between α-helices and β sheets to form a domain``
An enzyme has a mutation within the substrate binding site that reduces the binding of
the coenzyme needed for covalent catalysis. Which of the following is likely to result as
a consequence of this mutation? - Answers -The enzyme will not be able to form the
transition state complex
Domains are common structural elements that retain a particular function within the
protein. Which of the following is an example of a protein domain? - Answers -An
arrangement of β-strands connected by α-helices (βαβαβ)
Under low energy conditions, AMP will bind to phosphofructokinase I and glycogen
phosphorylase at site unique to the active site. This enhances the activity of these
enzymes. This is an example of which of the follow types of enzyme regulation? -
Answers -Allosteric activation
, If a mutation is made within the active site of an enzyme resulting in a decrease in Km,
which of the following will be true with respect to the enzyme kinetics? - Answers -The
enzyme will require a lower substrate concentration to reach ½Vmax`
A 28-year-old male is recently diagnosed with HIV. As part of his treatment he is
prescribed Nevirapine which interferes with retroviral reverse transcriptase enzyme.
Using the graph below, which of the following best describes the impact of this drug on
its target? - Answers -it reduces the catalytic activity of the enzyme
In the image below the blue line indicates enzyme kinetics with no inhibitor present.
Based on this information which of the following is true? - Answers -The green line
shows enzyme kinetics with the addition of a noncompetitive inhibitor
The following represents a series of reactions. As levels of B increase, this will decrease
the conversion of S3 to B. Which of the following best describes this type of regulation
in a biosynthetic pathway? - Answers -feedback inhibition
The association of DNA and histones can be modified by histone acetylation. A
decrease in histone acetylation will have which of the following impacts on the
association of DNA and histones? - Answers -increase DNA: histone association
Use the figure below to compare the activities of glucokinase and hexokinase. Based on
this information, which of the following is a true statement? - Answers -Glucokinase has
a higher Km than hexokinase.
An example of enzyme activation by cleavage is best illustrated by which of the
following? - Answers -Activation of chymotrypsinogen to chymotrypsin
The fully reduced quinol form of CoQ contains which of the following combinations of
electrons and protons? - Answers -2 electrons and 2 protons
A series of reactions have the following ΔG.
A + B --> C ΔG = -1
C + D --> E ΔG = +3
E + F --> G ΔG = -5
The overall ΔG for this series of reactions is determined to be which of the following
values? - Answers --3
Which of the following components of the electron transport chain passes electrons to
O2 and causing it to be reduced to H2O? - Answers -cytochrome c oxidase
Which of the following would be consistent with an exergonic reaction? - Answers -ΔG0
would be negative
The glycerophosphate shuttle is the major shuttle of most tissues. Which of the
following best describes the transfer of electrons by this shuttle? - Answers -Electrons
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