Exam (elaborations)
Histotechnology HTL Exam - Comprehensive Review Questions and Answers (Latest Update 2025)
- Course
- Institution
Histotechnology HTL Exam - Comprehensive Review Questions and Answers (Latest Update 2025)
[Show more]
Content preview
Histotechnology HTL Exam - ComprehensiveReview Questions and Answers (Latest
Update 2025)
Alcian Blue demonstrates - Correct Answer ✅Acid mucins
Perl's Prussian Blue shows - Correct Answer ✅Hemosiderin
Southgate's mucicarmine - Correct Answer ✅epithelial acid
mucins
Verhoeff's Van Gieson - Correct Answer ✅elastic
Van Kossa shows - Correct Answer ✅calcium
Ziehl Neelsen shows - Correct Answer ✅Acid fast bacilli
(T.B.)
Nuclei in Gomori's Aldehyde Fuchsin technique - Correct
Answer ✅yellow - no specific stain for nuclei
Mast cells in a Gomori's Aldehyde Fuchsin technique -
Correct Answer ✅purple - GAF not specific for elastic
Gram positive bacteria if over-differentiated after crystal
violet - Correct Answer ✅red
,Histotechnology HTL Exam - Comprehensive
Review Questions and Answers (Latest
Update 2025)
Nuclei in the Brown and Brenn Gram stain if over
differentiated with picric acid/acetone - Correct Answer
✅yellow
Fungi in the Periodic Acid Schiff (PAS) technique - Correct
Answer ✅magenta - chitin binds with Schiffs
Fungi in the Grocott's Methenamine Silver technique -
Correct Answer ✅black - silver impregnation
Neutral mucins in a PAS technique if potassium
permanganate is used as the oxidizing agent - Correct
Answer ✅light pink - colour of background - CHO
overoxidized from aldehydes to acid state
Cytoplasm in an H&E if over differentiated with acid alcohol -
Correct Answer ✅pink - only affects nuclei
Epithelial acid mucins in the Southgate's mucicarmine
technique - Correct Answer ✅red
Cytoplasm in an H&E if the blueing agent is not properly
removed - Correct Answer ✅very pale pink
,Histotechnology HTL Exam - Comprehensive
Review Questions and Answers (Latest
Update 2025)
Nuclei in the Congo Red technique - Correct Answer ✅blue
- stained with aluminum hematoxylin
Calcium in the Von Kossa technique - Correct Answer
✅black - silver impregnation
Glycogen, after diastase in the Periodic Acid Schiff technique -
Correct Answer ✅pale pink - background colour
Mycobacterium after Ziehl Neelsen technique - Correct
Answer ✅red - AFB stain with carbol fuchsin
Mycobacterium after Ziehl Neelsen technique - Correct
Answer ✅red - AFB stain with carbol fuchsin
Streptococcus after Ziehl Neelsen technique - Correct
Answer ✅blue - colour of background
Neutral lipids for Oil Red O cut on a cryostat - Correct
Answer ✅red to orange
Neutral lipids for Oil Red O cut from wax block - Correct
Answer ✅not demonstrated - removed
, Histotechnology HTL Exam - Comprehensive
Review Questions and Answers (Latest
Update 2025)
Hemosiderin after a Perl's Prussian Blue - Correct Answer
✅blue
Nuclei after Masson's trichrome using an aluminum
Hematoxylin - Correct Answer ✅red - same as cytoplasm
Fine elastic fibres in Verhoeff's Van Gieson (VVG) after leaving
in Van Gieson reagent too long - Correct Answer ✅not
black/not demonstrated - iron hematoxylin removed by the
acid
Cytoplasm if underdifferentiated in the VVG technique -
Correct Answer ✅grey to black
Reticulin fibres if iron alum is skipped in the Gordon and
Sweet's - Correct Answer ✅not demonstrated
Melanin if potassium permanganate was used as an oxidizing
agent in the Gomori Burtner technique - Correct Answer
✅not demonstrated - removed
Melanin in the Luxol fast blue if overdifferentiated with
lithium carbonate - Correct Answer ✅pink
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Allivia. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
62555 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now