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Exam of 6 pages for the course Aerobic Respiration at Aerobic Respiration (Respiration exam)
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Respiration examThe student closed the tap. After thirty minutes the drop of colored liquid had moved to
the left. Explain why the drop of colored liquid moved to the left?
(Practical number 9) - answer1) Oxygen taken up/used (by woodlouse). (change this
depending on type of question).
2) Carbon dioxide (given out) is absorbed by solution/potassium hydroxide.
3) Decrease/change in pressure.
What measurements should the student have taken to calculate the rate of aerobic
respiration in mm3 of oxygen g-1 h-1? * - answer1) Distance (drop moves) and time.
2) Mass of woodlouse.
3) Diameter/radius/bore of tubing/lumen/cross-sectional area.
DNP inhibits respiration by preventing a proton gradient being maintained across
membranes. When DNP was added to isolated mitochondria the following changes
were
observed
• less ATP was produced
• more heat was produced
• the uptake of oxygen remained constant.
Explain how DNP caused these changes? * - answer1) Less/no proton/H+ movement
so less/no ATP produced.
2) Heat released from electron transport/redox reactions/energy not used to produce
ATP is released as heat.
3) Oxygen used as final electron acceptor/combines with electrons (and protons).
labelling diagrams - answerlabelling diagrams
1) Glycolysis:
2) The Krebs cycle: - answer1) Cytoplasm.
2) Mitochondrion.
Apart from respiration, give three uses of ATP in a liver cell? - answer1) Source of
energy/of phosphate.
2) Active transport.
3) Phagocytosis.
4) Bile production.
5) Cell division / mitosis.
6) Synthesis of: glycogen; protein / enzymes; DNA / RNA; lipid / cholesterol; urea.
, Human skeletal muscle can respire both aerobically and anaerobically. Describe what
happens to pyruvate in anaerobic conditions and explain why anaerobic respiration is
advantageous to human skeletal muscle? * - answer1) Forms lactate; [extras - C2H5OH
/ CO2
- CANCEL]
Use of reduced NAD / NADH;
Regenerates NAD;
NAD can be re-used to oxidise more respiratory substrate / correct e.g./
allows glycolysis to continue;
Can still release energy/form ATP
when oxygen in short supply/when no oxygen;
In which part of the mitochondrion does the Krebs cycle take place? - answerMatrix.
Name two substances for which there would be net movement into the mitochondrion? -
answer1) pyruvate.
2) ADP; P / inorganic phosphate.
3) reduced NAD; oxygen.
The mitochondria in muscles contain many cristae. Explain the advantage of this? -
answer1) Larger surface area for electron transport chain / 2) More enzymes for ATP
production /oxidative phosphorylation.
3) Provide ATP for contraction.
Give two ways in which anaerobic respiration of glucose in yeast is similar to anaerobic
respiration of glucose in a muscle cell? * - answer1) ATP formed/used.
2) Pyruvate formed/reduced.
3) NAD/reduced NAD.
4) Glycolysis involved/two stage process.
Give two ways in which anaerobic respiration of glucose in yeast is different from
anaerobic respiration of glucose in a muscle cell? * - answerEthanol/alcohol formed by
yeast, lactate by muscle cell; CO2 released by yeast but not by muscle cell.
Each student repeated the experiment and the results were pooled. Explain the
advantages of collecting a large number of results? - answer1) Allows anomalies to be
identified.
2) Increases reliability of means/ results).
3) Allows use of statistical test.
If aerobic respiration had been investigated rather than anaerobic respiration, how
would you expect the volumes of gas collected at 30°C to differ from these results?
Explain answer? * - answerVolume(s) less/ no gas evolved;
Glucose has RQ of 1.0;
So (volume) CO2 evolved = (volume of) O2
taken in
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