Exam (elaborations)
ADVANCED BIOCHEM ACS EXAM QUESTIONS AND ANSWERS
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ADVANCED BIOCHEM ACS EXAM QUESTIONS AND ANSWERS....
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Coenzymes required by the pyruvate dehydrogenase multienzyme complex are -ANSWER Thiamine pyrophosphate, lipoic acid, Fad, CoA, & NAD+
A kinase is an enzyme that. - ANSWER transfers a phosphoryl group from
ATP
Which of these contains a thioester bond: - ANSWER succinyl CoA
In setting up an assay for measuring the total amount of an enzyme present in an
unknown sample, the substrate concentration should preferably be: - ANSWER
10 fold higher than Km
Dialysis would be most useful for the separation of: - ANSWER A ammonium
sulfate from an enzyme
An inhibitor that increases Km and does not alter the V max value is -
ANSWER A competitive inhibitor
Which amino acid side chain would not be expected would not be expected to
participate in covalent enzyme catalysis - ANSWER Leu
Which of the following statements is correct regarding the T(deoxy) and R(oxy)
states of tetrameric hemoglobin - ANSWER In the T to R transition, the central
, cavity of the tetramer shrinks in size preventing the allosteric modulator 2,3,-
biophosphylglycerate from binding
In the cell, when energy demands have been met, acetyl-CoA builds up and acts
as a positive allosteric effector for - ANSWER pyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is
normally varied, while keeping the others constant - ANSWER substrate
concentration
In a yeast cell growing aerobically on glucose, which would provide most of the
energy for growth? - ANSWER glycolysis to TCA cycle and oxidative
phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
ANSWER Reaction with excess B-mercaptoethanol at pH 8 in 6 M urea
The alpha helical secondary structure present in protein - ANSWER is
disrupted by proline, amine H and carbonyl O
What can you conclude about the thermodynamic spontaneity of a reaction that
has a negative value for delta H and positive for delta S - ANSWER The
reaction is spontaneous
The kinetics of most enzymatic reactions can be described in terms of Km and
Vmax. Which best describes Km? - ANSWER the substrate concentration
required to give half maximal velocity
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