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Samenvatting WC1b opdrachten student (1).docx
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Werkcollege 1b B09GEGOpdracht 1:
In onderstaand figuur staat een schematische tekening van een Cas9-sgRNA in complex met een
targetsite, verschillende fenotypes van en sequenties verkregen uit de nematode C. elegans na een
gene-editing experiment. Vier genen zijn ge-edit (lin-5, dpy-11, unc-119 en rol-1). Als bovenste rij
staat de wildtype sequentie. In blauw staat het sgRNA en in geel de PAM-site.
Figuur x: aangepast uit CRISPR/Cas9-targeted mutagenesis in Ceanorhabditis elegans, Waaier et al., Genetics 2013
a) Bekijk de data van lin-5 in de figuur. Waar (in het stukje sequentie van lin-5 dat is
weergegeven in de figuur) is de breuk exact gemaakt?
b) Zoek op waar in de genen (welk exon/intron) lin-5 en dpy-11 de breuk is gemaakt (gebruik de
wormendatabase: wormbase.org).
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