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ACS Biochemistry: Questions With Complete Solutions
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ACS Biochemistry: Questions With Complete Solutions
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ACS Biochemistry: Questions With Complete SolutionsNucleotide Right Ans - monomer of nucleic acids made up of a 5-carbon
sugar, a phosphate group, and a nitrogenous base
nitrogenous base Right Ans - adenine, guanine (2 hydrogen's) purines
thymine, cytosine, uracil (3 Hydrogen's) pyrimidines
Polymerase Chain Reaction Steps Right Ans - 1. *Denaturation* of the *DNA
sample* into *TWO STRANDS* 95c
2. *Anneal primers* to each Strand 50-60c
3. Copy each strand by a *heat-stable DNA POLYMERASE* 72c
4. *repeat cycle*
DNA ligase Right Ans - A linking enzyme essential for DNA replication;
catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5'
end of a growing chain.
Telomerase Right Ans - An enzyme that catalyzes the lengthening of
telomeres in eukaryotic germ cells.
Central Dogma Right Ans - DNA -> RNA -> Protein
RNA directionality Right Ans - 5' to 3'
DNA directionality Right Ans - one end has an exposed hydroxl group on
the 3' carbon of deoxiribose and the other end has an exposed phosphate
group on a 5' carbon
hydrogen bonding Right Ans - the intermolecular force in which a
hydrogen atom that is bonded to a highly electronegative atom is attracted to
an unshared pair of electrons of an electronegative atom in a nearby molecule
bonded to N, O or F
Bronsted acid Right Ans - donates a proton
, Lewis acid Right Ans - electron pair acceptor
dissociation constant Right Ans - an equilibrium constant between a ligand
and a protein, such as a receptor or an enzyme
Kw equation Right Ans - Kw=[H+][OH-]
pH equation Right Ans - pH = -log[H+]
pKa equation Right Ans - pKa = -logKa
Henderson-Hasselbalch equation Right Ans - pH = pKa + log [A-]/[HA]
Buffer Right Ans - compound that prevents sharp, sudden changes in pH
amino acids Right Ans - a simple organic compound containing both a
carboxyl (—COOH) and an amino (—NH2) group. that are chiral expect
glycine
How to determine the charge of amino acids at different pH's? Right Ans -
The isoelctric point of an amino acid is: Right Ans - The pH at which there
is no net electrical charge on the amino acid
Cation Right Ans - A positively charged ion
Anion Right Ans - A negatively charged ion
Using a positively charge column the _______ charged ions will elude first?
Right Ans - elude negatively charged molecules first
Using a negatively charge column the _______ charged ions will elude first?
Right Ans - elude positively charged molecules first
primary protein structure Right Ans - sequence of amino acids help
together by peptide bonds
secondary protein structure Right Ans - occurs when the sequence of
amino acids are linked by hydrogen bonds
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