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ACS Biochemistry First Semester – Questions With Correct Solutions
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ACS Biochemistry First Semester – Questions With Correct Solutions
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ACS Biochemistry First Semester – Questions WithCorrect Solutions
Buffer Order and Henderson Hasselbach Right Ans - Water, Buffer, Salt,
Base
pH = pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
Ramachandran plots Right Ans - Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance
Protein structure of a helix, B sheet, reverse turn Right Ans - a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix Right Ans - 3.6
Super secondary structure Right Ans - helix turn helix
parallel vs. antiparallel Right Ans - parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) Right Ans - pH at which a particular molecule carries
no net electrical charge.
Protein Separation Techniques (ALL) Right Ans - Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG
Protein Separation Techniques (H) Right Ans - Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) Right Ans - Salting out
, different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) Right Ans - Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) Right Ans - ion exchange
separation based on charge; + proteins stick to - beads
Protein Separation Techniques (SG) Right Ans - Size Exclusion/Gel
Filtration
separation based on size
Cuts (protein separation techniques) Right Ans - cut out what dont
need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)
Dialysis Right Ans - protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Electrophoresis Right Ans - gels - move by size with electric current
1. Isoelectric focusing - purely pH
2. SDS page
pH separation is by pI and charge in 2d
Protein sequencing (who cuts after what) Right Ans - Cyanogen bromide =
methionine
trypsin = K, and R
chymotrypsin = F, M, L, W, Y
IP Right Ans - Immunoprecipitation with proteins
1. add antibodies specific to protein of interest to lysed cells
2. add antibody binding protein on beads
3. centrifuge and protein is on bead with antibodies
4. gel elect
CO-IP Right Ans - observe protein protein interaction
Messelson Stalh Right Ans - RATSHIT.
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