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BIO 150 Lab Exam 1 Review
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BIO 150 Lab Exam 1 Review
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BIO 150 Lab Exam 1 Review 2024-2025A clean and organized laboratory work space is not a laboratory safety priority. -
ANSWER-False
Acid-fast staining procedure - ANSWER-1. Prepare smear
2. Apply ZN carbolfuchsin on top of paper
3. Steam for 5 minutes
4. Rinse with distilled water
5. Decolorize with acid-alcohol till runs clear,
6. Rinse with distilled water
7. Counterstain with methylene blue - 1 minute
8. Rinse with distilled water
9. Blot dry
Acid-fast staining results - ANSWER-A: ZN carbolfuchsin: Pink = Positive (+) acid-fast
B: Methylene blue: blue = Negative (-) non acid-fast
Aerotolerant anaerobes - ANSWER-Tolerate oxygen, but cannot use oxygen.
(Grow throughout medium.)
Agar concentration in semi-solid medium - ANSWER-0.4%
Agar concentration in typical nutrient agar - ANSWER-1.5%
Alternative names for reagents - ANSWER-Crystal violet - primary stain
Iodine - mordant
Alcohol/acetone solution - decolorizer
safranin - counter stain
Appropriate clothing for the laboratory includes sandals, tank tops, and shorts. -
ANSWER-False
Are acid-fast negative cells stained by carbolfuchsin? If so, how can this be a differential
stain? - ANSWER-Yes, carbolfuchsin stains all cells. Non acid-fast cells lose their color
when you apply acid/alcohol reagent; need to apply methylene blue counterstain.
Aseptic technique - ANSWER-Review page 28
Aspergillus - ANSWER-
Bacillus - ANSWER-rod shaped
Candida albicans (Yeast) - ANSWER-Most common form of candida (white)
, Categories of colony morphology - ANSWER--colony shape
-margin (edge)
-elevation
-texture
-color
Coccus - ANSWER-spherical shaped
Colony-forming unit (CFU) - ANSWER-refers to the number of colonies you count on a
dish
Cyanobacteria (Gloeocapsa) - ANSWER-
Cyanobacteria (Nostoc) - ANSWER-
Cyanobacteria (Oscillatoria) - ANSWER-
Endospore staining procedure - ANSWER-1. Prepare smear
2. Apply malachite green on top of paper
3. Steam for 5 min
4. Rinse with distilled water
5. Counterstain with safranin
6. Rinse with distilled water
7. Blot dry
Examples of simple stain - ANSWER-Crystal violet (purple)
Safranin (pink)
Methylene blue
Explain the purpose of steaming the slide during the staining process of endospores -
ANSWER-it forces the malachite green stain into the spore at a quicker rate, vs simply
applying to the slide for 15+ minutes in order for the spore to stain
Facultative anaerobes - ANSWER-Grow with oxygen or with no oxygen.
(Grow throughout medium, when O2 is present it will be denser at the top.)
Functions for each substance in gram staining - ANSWER-primary stain: colors ALL
cells purple
mordant: makes complex with crystal violet, creates large molecules, so stain can not
be removed
decolorizer: removes crystal violet from gram (-)ve cells
counterstain: stains gram (-)ve cells pink
Gram staining identification - ANSWER-Gram (+)ve cells: Purple in color
Gram (-)ve cells Pink in color
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