Exam (elaborations)
PCB 4522 Exam 2 Latest Fall/Spring Term % Guaranteed Pass (Complete)
- Course
- PCB 4522 Molecular Genetics.
- Institution
- Florida Atlantic University
PCB 4522 Exam 2 Latest Fall/Spring Term % Guaranteed Pass (Complete)
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PCB 4522 Exam 2 Latest Fall/SpringTerm 2024-2025 100% Guaranteed Pass
(Complete)
How does DnaA prevent reinitiation at the oriC until the next cell cycle?
a) DnaA binds to membrane phospholipids, and this inactivates its ATPase activity.
b) DnaA hydrolizes ATP, and this inactivates it.
c) DnaA binds ATP, and this inactivates it.
d) DnaA binds single-stranded DNA, and this inactivates it.
b) DnaA hydrolizes ATP, and this inactivates it.
Which is FALSE?
a) The palm is the most conserved domain in the DNA polymerase family.
b) DNA binds in the cleft between thumb and fingers.
c) DNA polymerases have a common structure of palm, fingers, thumb, exonuclease
and N-terminal domains.
d) The thumb domain is the most conserved in DNA.
d) The thumb domain is the most conserved in DNA.
DNA polymerase I Klenow fragment is NOT used for _________.
,a) Nick translation
b) Digesting away protruding 3'-overhangs
c) DNA sequencing
d) filling-in DNA at recessed 3'-ends
a) Nick translation
.
The DNA polymerase I Klenow fragment is missing _____________activity.
a) Endonuclease
b) 3' to 5' exonuclease
c) 5' to 3' DNA synthesis
d) 5' to 3' exonuclease
d) 5' to 3' exonuclease
Conditional mutations in which of the following would be classified as "€œslow-stop"?
a) DnaE
b) SSB
c) SeqA
d) DnaA and DnaG
d) DnaA and DnaG
Bacterial DNA polymerase I does NOT have ________ activity?
a) Endonuclease activity
b) 5' to 3' DNA synthesis activity
,c) 5' to 3' exonuclease activity
d) 3' to 5' exonuclease activity
a) Endonuclease activity
Tension in the bacterial supercoiled genome is relaxed by _____ ?
a) Primase
b) Terminal protein
c) Replicase
d) Topoisomerase
d) Topoisomerase
In eukaryotic DNA replication, which enzyme primes both DNA strands?
a) Gamma replicase
b) Delta replicase
c) Alpha primase
d) DNA polymerase beta
c) Alpha primase
Which both E. coli polymerases are used in translesion replication (SOS repair of
damaged DNA, e.g. repair of thymidine dimers)?
a) DNA polymerase V and III
b) DNA polymerase IV and V
, c) DNA polymerase II and III
d) DNA polymerase IV and I
b) DNA polymerase IV and V
Which helices of DNA polymerase rotate 60 degrees to recruit the incoming dNTP?
a) Helices of the exonuclease domain
b) O-helixes of the finger domains
c) R helices of the palm domain
d) H helices of the thumb domain
b) O-helixes of the finger domains
.
Which answer is FALSE?
a) DnaB propels the PhiX174 phage leading strand synthesis replication fork.
b) DnaA binds to short repeated sequences at oriC.
c) Six monomers of DnaC bind each hexamer of DnaB.
d) DnaB is a hexameric protein that hydrolyzes ATP.
a) DnaB propels the PhiX174 phage leading strand synthesis replication fork.
Which activity of DNA polymerase I provides proofreading during DNA replication?
a) Endonuclease activity
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