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Samenvatting bacteriologie
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BACTERIOLOGIE2023-2024
FARMACEUTISCH EN BIOLOGISCHE LABORATORIUM TECHNOLOGIEN
,Lowie Boels Bacteriologie
1. ............................................................................................................................................3
1.1.......................................................................................................................................3
2. Micro-organisme in onderzoek .........................................................................................4
2.1 Introduction .............................................................................................................4
2.2 De humane microbiota.............................................................................................4
1. Eigenschappen ........................................................................................................4
2. Ontwikkeling van de microbiota ................................................................................5
3. Rol van de microbiota in gezondheid .........................................................................6
4. Rol van microbiota in ziekte ......................................................................................7
5. Enterotypes .............................................................................................................8
2.3 Microbiële detectie technieken .................................................................................9
1. Cultuur afhankelijke methode ..................................................................................9
2. Cultuur onafhankelijke methode............................................................................. 10
3. Interpretatie van diversiteit in de microbiota ........................................................... 12
2.4 Onderzoek in de microbiota van tegenwoordig ........................................................ 12
1. Humane studies .................................................................................................... 12
2. Dieren modellen .................................................................................................... 13
3. In vitro-simulatoren................................................................................................ 14
2.5 Modulatie strategieën van de microbiota................................................................. 16
1. Pre en pro biotica ................................................................................................... 16
2. Symbiotica ............................................................................................................ 18
3. Postbiotica ............................................................................................................ 18
4. Psychobiotica ........................................................................................................ 18
5. Levende bio-therapeutische producten .................................................................. 19
6. Fecale microbiota transplantatie ............................................................................ 19
7. Antibiotica ............................................................................................................. 22
8. Antibiotica resistentie ............................................................................................ 28
1) Glycopeptide resistentie ........................................................................................ 30
2.6 Biofilms ................................................................................................................. 34
1. Wat zijn biofilms..................................................................................................... 34
2. Waarom worden biofilms gevormd? ................................................................... 36
3. Hoe word een biofilm gevormd? ......................................................................... 37
4. biofilm gerelateerde ziektes ............................................................................... 38
5. Biofilm resitentie tegen antibiotica ..................................................................... 39
6. Biofilm inhibitie ................................................................................................. 40
3. Micro-organisme in industrie ...................................................................................... 42
1
,Lowie Boels Bacteriologie
3.1. Voedsel industrie ................................................................................................... 42
1. Voedsel productie ............................................................................................. 42
2. Parameters die microbiële groei beïnvloeden .......................................................... 44
3. Bewaring ............................................................................................................... 45
4. Voedselveiligheid ................................................................................................... 46
3.2. Farmaceutische industrie: steriliteit en veiligheid .................................................... 49
1. Steriliteit testen.................................................................................................. 49
2. Most probable number test (MPN) ...................................................................... 53
3. LAL test = limulus amoebocyte lysaat test .......................................................... 55
4. Ames test .......................................................................................................... 59
3.3. Farmaceutische industrie: bacteriocines ................................................................ 61
1. Inleiding ............................................................................................................. 61
2. Classificatie van bacteriocines ........................................................................... 61
3. Actiemechanismes ................................................................................................ 65
4. Applicaties......................................................................................................... 65
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, Lowie Boels Bacteriologie
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