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BICH 431 Exam 1 Questions and Answers
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BICH 431 Exam 1 Questions and Answers
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BICH 431 Exam 1 Questions andAnswers
Replication errors occur every - Answer-10^8 bases, corrected to 10^10
Mutagenesis - Answer-increasing the likelihood that the desired mutation will happen
Directed Mutagenesis - Answer-introducing designed changes into chromosomal DNA-
ex. Point mutations, gene fusions, deletions or knockouts
Gene Fusions - Answer-fuse protein to a reporter gene to track it in the cell
Knockouts - Answer-Deleting a gene and then seeing what happens
Forward Genetics - Answer-starting with a phenotype and work your way forward to
identify underlying genetics
Reverse genetics - Answer-starting with genes and looking for phenotype
What size of DNA fragments is used in agarose gels? - Answer-150 bp to genomic size
What size DNA fragments are used in acrylamide gels? - Answer-high resolution, down
to 1bp
Which form of DNA is the slowest to migrate? Nicked, linear, or supercoiled? - Answer-
nicked
Which form of DNA is fastest to migrate? - Answer-Supercoiled
What are the components of a sequencing reaction? - Answer-Primer (1) dNTP's,
polymerase, ddNTP's, buffer, template sequence
Southern Blot - Answer-Detects abundance of DNA and size, and can be used to detect
specific sequence using a probe
Northern Blot - Answer-detects abundance and size of RNA, similar to a southern but
the RNA must be denatured out of secondary structure
Which strand is extended? - Answer->
At what stage is DNA replication regulated? - Answer-Initiation for both bacteria and
eukaryotes. After Initiation, replication will proceed at a steady rate until the
chromosome is replicaed
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