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Bio 431-Exam2-Immune system Questions and Answers
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Bio 431-Exam2-Immune system Questions and Answers
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BIOC 431 Exam 1 Questions andAnswers
Vasopressin (Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Arg-Gly) is critical in regulating water
reabsorption in the kidneys. If chymotrypsin & trypsin are added, what would fragments
be?
a. Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Arg Gly
b. Cys-Tyr Phe Gln-Asn-Cys-Pro-Arg-Gly
c. Cys Tyr Phe-Gln-Asn-Cys-Pro-Arg-Gly
d. Cys-Tyr Phe Gln-Asn-Cys-Pro-Arg Gly - Answer-d
What would be a good method for desalting your protein?
a. dialysis
b. column chromatography
c. both
d. none - Answer-c
Which method breaks open bacterial cells with high pressure?
a. french press
b. sonication
c. high pressure liquid chromatography
d. centrifugation - Answer-a
worked with oligopeptides,
sequencing with proteins
sequencing with DNA sequences/nucleic acids - Answer-Sanger
PITC to N-terminal amino acid and then treated with TFA
peptide is cleaved between the first and second amino acid
protein cleavage - Answer-Degradation
proteins longer than 50 residues need - Answer-protein cleavage
cleaves on the c-side of Lys and Arg - Answer-trypsin
Cleaves at C-side of tyr, trp, and phe - Answer-chymotrypsin
-measures the mass of small peptide fragments
-measures the mass to change ratio
-can be used to determine the molecular mass - Answer-Mass spectrometry
-releases polypeptides that have been cleaved by trypsin via a solvent in gas phase
-this produces highly charged molecules that fly in an electric field in a vaccuum -
Answer-electrospray ionization
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