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MMG 431 Exam 1 Questions and Answers
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MMG 431 Exam 1 Questions and Answers
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MMG 431 Exam 1 Questions andAnswers
beta-clamp - Answer-hold DNA polymerase on
ter - Answer-assists in the directionality of DNA sequence
Tus - Answer-protein that binds to ter sites; knocks off helicase
DnaA - Answer-binds to oriC and begins replication
Hda - Answer-protein interacts with beta clamp on lagging strand to generate DnaA-
ADP
DARS - Answer-DnaA activating sequences; convert DnaA-ADP to DnaA-ATP
dam - Answer-responsible for methylating DNA strands (GATC sequence); high basal
mutation rate if deleted
SeqA - Answer-binds to hemimethylated oriC and blocks initiation
Mfd - Answer-removes RNAP from DNA during collision with DNAP
Ftsk - Answer-pump that moves DNA in one direction; directionality determined by
KOPS sites
xerCD - Answer-interacts with Ftsk at septum
oriC - Answer-origin of chromosome
; when initiation always happens)
oriT - Answer-origin of transfer; necessary for transfer of DNA during bacterial
conjugation
oriV - Answer-origin of vegetative replication; necessary for plasmids
DUE - Answer-DNA unwinding element; unwinds DNA, allowing DnaA to bind with itself
and creates single stranded DNA
DnaC - Answer-binds to single stranded DNA at DUE and recruits DnaB
DnaB - Answer-interacts with only one strand; one found on each DNA strand in
opposite directions
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