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MMG 431 EXAM 3 Questions and Answers
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MMG 431 EXAM 3 Questions and Answers
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MMG 431 EXAM 3 Questions andAnswers
oriV - Answer-origin of replication of plasmids. A cis element.
oriT - Answer-a second origin found in transmissible plasmids
stringent copy number - Answer-low or fixed copy number
relaxed copy number - Answer-high copy number
Two ways for plasmid replication - Answer-1) Unidirectional replication
2) Bidirectional replication
Rolling circle replication (sigma replication) - Answer-there are two types of sigma
replication: SSO and DSO. you can have single strand replication or double strand
replication.
Cured cell - Answer-Cells that do not have a single copy of the plasmid.
How to enhance curing of plasmids - Answer-Adding EtBr or proflavine to the medium
Theta replication - Answer-regular replication. there are two strands involved.
RNA 2 - Answer-initiates replication of the plasmid. It interacts with RNase H to begin
initiation.
RNA 1 - Answer-Binds to RNA 2 and interacts with Rop and forms a kissing complex,
preventing the initiation of replication of the plasmid.
Lederberg and the Tatum experiment - Answer-showed that sexual recombination
occurs between cells of E. coli. They observed recombinant types when two auxorophic
mutants were mixed.
Shuttle vectors - Answer-allow plasmids to replicate in more diverse organisms
Persister cells - Answer-persister cells have more toxins, so that growth is inhibited, and
this results in the cell not dying due to the antibiotics.
mazEF toxin/ antitoxin system - Answer-mazEF cleaves rna at certain sites
mazE - Answer-antitoxin
mazF - Answer-toxin
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