Summary
Summary Advanced fluoresence: overview of all the techniques
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this excel sheet contains all the techniques discussed in the course, along with some characteristics and what to use when.
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Microscopy Types of microscopescharacteristics Transmitted light/bright field microscopy
(upright conformation)
Epi-illumination microscopy
(upright conformation)
Light sources
Mercury Arc lamp
Tungsten Halogen lamp
LEDs
Lasers
Detectors
Area detectors Charge-coupled device
(CCD)
Complementary metal
oxide semiconductor
(CMOS)
Point detectors Photomultiplier tube
(PMT)
, Point detectors Photomultiplier tube
(PMT)
Gallium arsenide
phosphide (GaAsP)
Avalanche photodiode
Zeiss Airy scan
Filters
Neutral density filters
Color filter
Dichromatic filter (dichroic mirror)
Microscopy Optical microscopy (transmitted light microscopy)
techniques Bright field microscopy
Dark field microsopy
,Dark field microsopy
Phase contrast microscopy
Polarization microscopy
Differential interference contrast (DIC)
microscopy
Fluorescence microscopy (background free signal detection)
Epi-fluorescence microscopy (widefield)
Confocal laser scannng microscopy (CLSM)
, Confocal laser scannng microscopy (CLSM)
Total internal reflection fluorescence
microscopy (TIRFM)
Two-Photon excitation microscopy (TPEM)
confocal
Spinning disk confocal microscopy (Nipkow
Disk)
Selective plane illumination microscopy
(SPIM)
Single molecule fluorescence (Single
particle tracking SPT)
Super Single molecule
resolution: localization microscopy
overcome (SMLM)
diffraction limit
(200-300 nm)
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