What are the differences between microtubules, intermediate filaments and actin
filaments? - -Actin: responsible for have and movement; single subunit
-Intermediate: keeping cells whole and attached; proteins many types
-Microtubules: highway: carries vesicles, chromosomes, etc across membrane;
alpha and beta tublin proteins
What is each type of filament made of? - -Actin: actin subunits, one type
-Intermediate: many types of proteins
-Microtubules: made of alpha and beta tublin dimers
What are the functions of each type of filament? - -Microtubule: highways, motor
proteins pull cargo along microtubule
-Actin: responsible for shape and movement
-Intermediate: keeping cells whole and attached
Where in the cell would you find each type of filament? - Actin is underneath the
cell membrane, microtubules radiate out from the centrosome, and intermediate
filaments cross link the cell.
, Understand polymerization of actin and microtubules - Microtubules bind GTP for
polymerization and require a GTP cap to grow. The ARP complex nucleates actin
filaments. ATP is required for actin.
What is the difference between the plus and the minus end of actin and
microtubules? - Both grow at the (+) end, but GTP is bound in microtubules and
ATP in actin.
What are GTP caps? What type of filaments have these caps? - -Microtubules
have these caps
- positive GTP cap, microtubule grows
-If GTP cap is lost, microtubule shrinks
-If GTP cap is regained, growth restarts
-How many subunits with GTP are available
What are GTP caps? What types of filaments have these caps? - GTP cap grows
microtubule, if GTP cap is lost, microtubule shrinks. If the GTP cap is regained,
growth restarts
Compare the mechanical properties of actin, microtubules and intermediate
filaments. - Intermediate filaments can stand deformation and deforming force.
Microtubules and actin filaments cannot.
Name an intermediate filament. - Keratin
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