Exam (elaborations)
Bios 344 - Exam 3 Questions answers latest update
- Course
- BIOS 34344
- Institution
- University Of Notre Dame
Bios 344 - Exam 3 Questions answers latest update
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Bios 344 - Exam 3genetic map - recombination frequencies
cytological map - staining patterns of bands
physical map - bp distances, actual sequences
RFLP Analysis steps - isolate, digest, separate on gel, nylon membrane, hybridize
on Southern with radioactive probes, wash
VNTRs - variable number of tandem repeats - very polymorphic, have different
amounts, often noncoding regions
STRs - short tandem repeats - 2-5 nts, microsatellites
FISH - fluorescent in situ hybridization - used VNTRS and STRs as hybridization
probes to position a probe on a cytological map
average kb between genes - 145kb
average gene - 14,000 bp ling, 7-9 exons
, exon percentage - 1.1
intron percentage - 24
intergenic percentage - 75
LINEs - long interspersed elements - intergenic, encode functional reverse
transcriptase transposons
SINEs - Short interspersed elements— have internal RNA promoter, ALU
sequence, a group of non-reverse-transcriptase-encoding retrotransposons.
noncoding functional elements - promoters, enhancers, centromeres, telomeres
haplotype - 2 or more single nt polymorphisms that are close enough together
that the alleles don't separate with recomb very often
microarrays / gene chips - allow whole genotype analysis
steps: mRNA --> RT PCR --> cDNA, array has probes for every gene, cDNA labeled,
allow to hybridize, see colors (red, green, yellow for both)
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