Microbio 150 Lab exam 1 PVCC
Questions And Answers.
Always - Verified ANS ✔✔ - When preparing to view a specimen on a
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compound microscope in the lab, one should __________ begin with the 4x
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objective
ml
In the bio-hazard waste container - Verified ANS ✔✔ - Where should used
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gloves be disposed of in the microbiology lab
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4x - Verified ANS ✔✔ - Before putting a dust cover over the microscope and
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placing it in the cabinet, which objective lens should be rotated over the stage
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Condenser lens - Verified ANS ✔✔ - On the microscope, what concentrates the
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light on the specimen?
ml ml ml ml
Objective lens - Verified ANS ✔✔ - On the microscope, what bends light
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passing through the specimen to create a real image
ml ml ml ml ml ml ml ml ml
Iris diaphragm - Verified ANS ✔✔ - On the microscope, what allows for more or
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less light to be directed onto the specimen
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Resolution - Verified ANS ✔✔ - On the microscope, what gives you clarity of
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the image
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To ensure it is sterilized, done so no cross contamination happens - Verified ANS
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✔✔ - Why is desiccating your inoculating loop important? When should this be
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done?
ml
Before and after dipping loop in - Verified ANS ✔✔ - During an aseptic transfer,
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when do you pass the mouth of the test tube through the flame?
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Loop, broth - Verified ANS ✔✔ - During the broth to slide aseptic transfer, a
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__________ was used to obtain a pure culture from a __________.
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, Attach it firmly to the slide - Verified ANS ✔✔ - Prior to staining, smears of
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microorganisms are heat-fixed in order to
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Inoculation - Verified ANS ✔✔ - The term that refers to the purposeful addition
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of microorganisms into a nutrient medium is
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False - Verified ANS ✔✔ - True or false: In the microbiology lab, it is acceptable
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to move your loop around while waiting for it to dry.
ml ml ml ml ml ml ml ml ml ml ml
Nigrosin - Verified ANS ✔✔ - Which stain was used during the negative staining
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procedure?
ml
Methylene blue - Verified ANS ✔✔ - Which stain was used during the simple
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
staining procedure?
ml ml
False - Verified ANS ✔✔ - True or false: During a negative stain, the bacteria
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
are heat fixed.
ml ml ml
Peptidoglycan - Verified ANS ✔✔ - Bacteria in the Mycobacterium genus have ml ml ml ml ml ml ml ml ml ml ml ml ml
the presence of what component in the cell wall that causes them to be acid-
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
fast?
Purple; colorless - Verified ANS ✔✔ - After the decolorizer has been added,
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gram-positive organisms appear __________ and gram-negative organisms
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appear __________.
ml ml
It forms a complex with crystal violet - Verified ANS ✔✔ - Why is iodine (or a
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mordant) used in the Gram staining process?
ml ml ml ml ml ml ml
False - Verified ANS ✔✔ - True or false: bacterial slides are not heat-fixed prior
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
to beginning to Gram staining.
ml ml ml ml ml
The cap should be held in the pinky of your dominant hand - Verified ANS ✔✔ -
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When completing a bacterial transfer from a broth, where should the cap be
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located while you are inserting your inoculating instrument in the bacterial
ml ml ml ml ml ml ml ml ml ml ml
growth?
ml
Agar slant - Verified ANS ✔✔ -
ml ml ml ml ml ml ml ml ml
Broth tube - Verified ANS ✔✔ -
ml ml ml ml ml ml ml ml ml
Agar plate - Verified ANS ✔✔ -
ml ml ml ml ml ml ml ml ml
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