Microscope- The Condenser - (correct answer) -focuses the light ging into the
ml ml ml ml ml ml ml ml ml ml ml
objective lens
ml ml
Microscope- The Iris Diaphragm - (correct answer) -adjusts the amount of light
ml ml ml ml ml ml ml ml ml ml ml
going into the objective lens
ml ml ml ml ml
Microscope- Nosepiece - (correct answer) -holds the objective lenses and rotates
ml ml ml ml ml ml ml ml ml ml
to change from one objective to another
ml ml ml ml ml ml ml
Microscope- Coarse Objective Knob - (correct answer) -moves the stage larger
ml ml ml ml ml ml ml ml ml ml
distances to help get the object in focus
ml ml ml ml ml ml ml ml
Microscope- Fine Adjustment Knob - (correct answer) -moves the stage smaler
ml ml ml ml ml ml ml ml ml ml
increments to help get the object in better focus
ml ml ml ml ml ml ml ml ml
Microscope- Objective Lens - (correct answer) -magnifies the object. It is located
ml ml ml ml ml ml ml ml ml ml ml
near the object.
ml ml ml
Microscope- Occular Lens - (correct answer) -magnifies the image of the object
ml ml ml ml ml ml ml ml ml ml ml
from the objective lens. It is located near the eye.
ml ml ml ml ml ml ml ml ml ml
Steps in using the microscope - (correct answer) -1) scanner power in place
ml ml ml ml ml ml ml ml ml ml ml ml
2) place slide of specimen stage in place with stage clips
ml ml ml ml ml ml ml ml ml ml
3) Using the course adjustment knob, raise the stage all the way up and then
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down slowly until you can see image of specimen
ml ml ml ml ml ml ml ml ml
4) Readjust with fine adjustment knob
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Describe the purpose of the oil immersion lens - (correct answer) -Used when oil
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is added to the slide. Oil must touch the object and objective lens to that the
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
light does not refract futher
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, Parfocal - (correct answer) -Parfocal microscopes are able to move from one
ml ml ml ml ml ml ml ml ml ml ml
objective to the next and still be in focus due to the length of the objective
ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml
lenses
ml
Resolution - (correct answer) -The ability to distinguish between two objects that
ml ml ml ml ml ml ml ml ml ml ml
are very close together.
ml ml ml ml
What is resolving power? - (correct answer) -The measurement of resolution
ml ml ml ml ml ml ml ml ml ml
What are two ways to increase resolving power? - (correct answer) -1. By
ml ml ml ml ml ml ml ml ml ml ml ml
decreasing the wavelength of light (using a blue filter) 2. Increasing the
ml ml ml ml ml ml ml ml ml ml ml ml
numerical aperature
ml ml
What units are used to express resolving power? - (correct answer) -nm or
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nanometers
ml
What is better, smaller or larger resolving power? - (correct answer) -Smaller is
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better
ml
Light microscopes have a 200nm resolving power
ml ml ml ml ml ml
Electron microscopes have a .2nm resolving power
ml ml ml ml ml ml
What is chromatic aberration? - (correct answer) -The effect when many colors
ml ml ml ml ml ml ml ml ml ml ml
are seen toward the edge of the microscope field of vision (like a prisim)
ml ml ml ml ml ml ml ml ml ml ml ml ml ml
How are microscopes corrected for chromatic aberration? - (correct answer) -
ml ml ml ml ml ml ml ml ml ml
With a special achromatic lens (corrected for red and blue light) or by using a
ml ml ml ml ml ml ml ml ml ml ml ml ml ml
blue filter. The lab has blue filters in their microscopes which also increase
ml ml ml ml ml ml ml ml ml ml ml ml ml
resolution.
ml
Describe the process of making a wet-mount slide - (correct answer) -1) Add the
ml ml ml ml ml ml ml ml ml ml ml ml ml
specimen to the slide
ml ml ml ml
2) Touch the edge of the cover slip with one edge of drop of specimen.
ml ml ml ml ml ml ml ml ml ml ml ml ml ml
3) Let the cover slip drop gradually to the slide, covering the drop of specimen.
ml ml ml ml ml ml ml ml ml ml ml ml ml ml
Describe the purpose of making a wet-mount slide - (correct answer) -To be able
ml ml ml ml ml ml ml ml ml ml ml ml ml
to see living organisms under the microscope in their natural state.
ml ml ml ml ml ml ml ml ml ml ml
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