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2024/2025 MICROBIOLOGY LAB EXAM 1 WITH CORRECT ANSWERS
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2024/2025 MICROBIOLOGY LAB EXAM 1 WITH CORRECT ANSWERS
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2024/2025 MICROBIOLOGY LAB EXAM 1WITH CORRECT ANSWERS
What is resolving power? - CORRECT ANSWERS- The measurement of
resolution
What are two ways to increase resolving power? - CORRECT ANSWERS- 1. By
decreasing the wavelength of light (using a blue filter) 2. Increasing the
numerical aperature
What units are used to express resolving power? - CORRECT ANSWERS- nm
or nanometers
What is better, smaller or larger resolving power? - CORRECT ANSWERS-
Smaller is better
Light microscopes have a 200nm resolving power
Electron microscopes have a .2nm resolving power
Microscope- The Condenser - CORRECT ANSWERS- focuses the light ging
into the objective lens
Microscope- The Iris Diaphragm - CORRECT ANSWERS- adjusts the amount
of light going into the objective lens
Microscope- Nosepiece - CORRECT ANSWERS- holds the objective lenses and
rotates to change from one objective to another
,Microscope- Coarse Objective Knob - CORRECT ANSWERS- moves the stage
larger distances to help get the object in focus
Microscope- Fine Adjustment Knob - CORRECT ANSWERS- moves the stage
smaler increments to help get the object in better focus
Microscope- Objective Lens - CORRECT ANSWERS- magnifies the object. It is
located near the object.
Microscope- Occular Lens - CORRECT ANSWERS- magnifies the image of the
object from the objective lens. It is located near the eye.
Steps in using the microscope - CORRECT ANSWERS- 1) scanner power in
place
2) place slide of specimen stage in place with stage clips
3) Using the course adjustment knob, raise the stage all the way up and then
down slowly until you can see image of specimen
4) Readjust with fine adjustment knob
Describe the purpose of the oil immersion lens - CORRECT ANSWERS- Used
when oil is added to the slide. Oil must touch the object and objective lens to
that the light does not refract futher
Parfocal - CORRECT ANSWERS- Parfocal microscopes are able to move from
one objective to the next and still be in focus due to the length of the
objective lenses
, Resolution - CORRECT ANSWERS- The ability to distinguish between two
objects that are very close together.
What is chromatic aberration? - CORRECT ANSWERS- The effect when many
colors are seen toward the edge of the microscope field of vision (like a
prisim)
How are microscopes corrected for chromatic aberration? - CORRECT
ANSWERS- With a special achromatic lens (corrected for red and blue light)
or by using a blue filter. The lab has blue filters in their microscopes which
also increase resolution.
Describe the process of making a wet-mount slide - CORRECT ANSWERS- 1)
Add the specimen to the slide
2) Touch the edge of the cover slip with one edge of drop of specimen.
3) Let the cover slip drop gradually to the slide, covering the drop of
specimen.
Describe the purpose of making a wet-mount slide - CORRECT ANSWERS- To
be able to see living organisms under the microscope in their natural state.
What is the advantage of using a wet-mount slide? - CORRECT ANSWERS-
Can see movement and determine if microbes posses their own method of
motility. Can also see the actual shape and arrangement of microbes.
What is the dis-advantage of using a wet-mount slide? - CORRECT ANSWERS-
The movement is very fast, microbes are transparent. Must use a "dark-field
stop".
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