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In Clinical Laboratory Science Bacteriology – Mycobacteria Questions and Answers Already Passed

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In Clinical Laboratory Science Bacteriology – Mycobacteria Questions and Answers Already Passed What is the main feature used to differentiate Mycobacterium species in a clinical laboratory? Acid-fastness Colony shape and size Gram stain reaction Motility Which medium is commonl...

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  • December 9, 2024
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  • 2024/2025
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  • In Clinical Laboratory Science Bacteriology –
  • In Clinical Laboratory Science Bacteriology –
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In Clinical Laboratory Science
Bacteriology – Mycobacteria Questions
and Answers Already Passed
What is the main feature used to differentiate Mycobacterium species in a clinical laboratory?


✔✔Acid-fastness


Colony shape and size

Gram stain reaction

Motility




Which medium is commonly used to grow Mycobacterium species in the laboratory?

Blood agar

Chocolate agar


✔✔Lowenstein-Jensen medium


MacConkey agar




What does the term "acid-fast" refer to in the laboratory identification of Mycobacteria?

The ability to retain dye after treatment with acid alcohol

The ability to grow on acid media


1

,The ability to form spores in acidic conditions

The ability to resist being killed by acidic pH




Which is the most commonly used method to confirm the presence of Mycobacterium

tuberculosis in clinical samples?


✔✔Acid-fast bacilli (AFB) smear and culture


Polymerase chain reaction (PCR)

Blood culture

Lateral flow immunoassay




Which of the following is a common non-tuberculous Mycobacterium species found in clinical

specimens?

Mycobacterium bovis

Mycobacterium leprae


✔✔Mycobacterium avium complex


Mycobacterium tuberculosis




In the clinical laboratory, which of the following is a critical aspect of isolating Mycobacteria

from clinical specimens?

2

, ✔✔Incubating at 37°C in a CO2-enriched environment


Incubating at room temperature with exposure to UV light

Incubating in a deep freezer

Incubating at 45°C in an aerobic environment




What staining method is typically used to detect Mycobacterium tuberculosis in clinical sputum

samples?


✔✔Ziehl-Neelsen stain


Wright-Giemsa stain

Gram stain

Löffler's methylene blue stain




Which test is used in the clinical laboratory to assess Mycobacterium tuberculosis resistance to

rifampin?


✔✔GeneXpert MTB/RIF assay


Lowenstein-Jensen culture

Acid-fast staining

Molecular beacon assay



3

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