Microbiology Lab Final Exam Questions and Answers 100% Pass
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Course
Microbiology Lab
Institution
Microbiology Lab
Microbiology Lab Final Exam Questions
and Answers 100% Pass
What does a pure culture contain?
A single type of microorganism.
Why is the inoculating loop sterilized before use?
To eliminate any contaminating organisms.
What is the first step in preparing a bacterial smear?
Applying a...
Microbiology Lab Final Exam Questions
and Answers 100% Pass
What does a pure culture contain?
✔✔A single type of microorganism.
Why is the inoculating loop sterilized before use?
✔✔To eliminate any contaminating organisms.
What is the first step in preparing a bacterial smear?
✔✔Applying a small drop of water to the slide.
What does the acid-fast stain detect?
✔✔Bacteria with waxy cell walls, such as Mycobacterium.
What is the purpose of incubation in bacterial culture growth?
✔✔To provide optimal conditions for microbial growth.
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,What does colony morphology help identify?
✔✔The type of microorganism based on size, shape, and color of colonies.
Why are agar plates stored upside down during incubation?
✔✔To prevent condensation from dripping onto the surface.
What is the role of the decolorizer in the Gram stain?
✔✔To remove the primary stain from Gram-negative cells.
Why is blood agar used in microbiology labs?
✔✔To detect hemolytic activity of bacteria.
What type of organism grows only in the absence of oxygen?
✔✔Obligate anaerobe.
What is the streak plate method used for?
✔✔Isolating individual bacterial colonies from a mixture.
2
, What does a catalase test detect?
✔✔The production of the enzyme catalase, which breaks down hydrogen peroxide.
What is the significance of a positive oxidase test?
✔✔It indicates the presence of cytochrome c oxidase in bacteria.
Why are biochemical tests performed on bacteria?
✔✔To identify specific metabolic or enzymatic activities.
What is the purpose of using nutrient agar?
✔✔To support the growth of a wide variety of microorganisms.
What is the term for bacteria that thrive in high salt concentrations?
✔✔Halophiles.
What color do acid-fast bacteria stain?
✔✔Red or pink.
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