Microbiology Lab - Exam 2 Questions and Answers 100% Pass
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Microbiology Lab -
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Microbiology Lab -
Microbiology Lab - Exam 2 Questions
and Answers 100% Pass
What is the purpose of the aseptic technique during inoculation?
To prevent contamination of the sample and maintain pure cultures.
What is the function of the autoclave in a microbiology lab?
To sterilize equipment and media usin...
Microbiology Lab - Exam 2 Questions
and Answers 100% Pass
What is the purpose of the aseptic technique during inoculation?
✔✔To prevent contamination of the sample and maintain pure cultures.
What is the function of the autoclave in a microbiology lab?
✔✔To sterilize equipment and media using high pressure and steam.
What is the color of Escherichia coli colonies on MacConkey agar?
✔✔Pink, indicating lactose fermentation.
What is the morphology of Vibrio bacteria?
✔✔Comma-shaped.
What is the primary reason for using a control organism in biochemical tests?
✔✔To verify the accuracy and validity of the test results.
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,What does a positive citrate test indicate?
✔✔The organism can utilize citrate as its sole carbon source.
What is the appearance of Mycobacterium in an acid-fast stain?
✔✔Red or pink rods.
What is the role of iodine in the Gram stain procedure?
✔✔To act as a mordant that fixes the crystal violet to the bacterial cell wall.
What does a positive indole test result look like?
✔✔A red or pink layer on top of the medium after adding Kovac's reagent.
What is the purpose of using phenol red in carbohydrate fermentation tests?
✔✔To detect acid production based on a color change.
What does a black precipitate in a TSI (triple sugar iron) agar test indicate?
✔✔The production of hydrogen sulfide.
2
,What is the term for bacteria that grow only in the presence of oxygen?
✔✔Obligate aerobes.
What is the appearance of a positive gelatinase test?
✔✔The gelatin medium becomes liquefied.
What is the significance of resolving power in microscopy?
✔✔It determines the clarity and detail visible in the specimen.
What does a positive methyl red test indicate?
✔✔The production of stable acids from glucose fermentation.
What is the primary purpose of using a control plate when inoculating bacterial cultures?
✔✔To ensure that the culture medium is sterile before inoculation.
What does a bubbling reaction in the catalase test signify?
✔✔The presence of the enzyme catalase, which breaks down hydrogen peroxide.
3
, What is the main purpose of the urea hydrolysis test?
✔✔To determine if the organism produces urease, which hydrolyzes urea.
What is the function of the fine adjustment knob on a microscope?
✔✔To achieve a sharper focus on the specimen.
What is the purpose of using thioglycollate broth in microbiology?
✔✔To test the oxygen requirements of microorganisms.
What is the term for bacteria that grow best at high temperatures?
✔✔Thermophiles.
What is the main function of bacterial endospores?
✔✔To ensure survival under adverse environmental conditions.
What is the purpose of a wet mount preparation?
✔✔To observe live microorganisms and assess their motility.
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