Microbio 150 Lab Exam 1 PVCC
Questions and Answers Already Passed
What is the primary purpose of performing a Gram stain in the lab?
To classify bacteria as Gram-positive or Gram-negative based on their cell wall structure.
What is the name of the dye used as the primary stain in the Gram s...
Microbio 150 Lab Exam 1 PVCC
Questions and Answers Already Passed
What is the primary purpose of performing a Gram stain in the lab?
✔✔To classify bacteria as Gram-positive or Gram-negative based on their cell wall structure.
What is the name of the dye used as the primary stain in the Gram staining process?
✔✔Crystal violet.
What is the function of the mordant in the Gram stain procedure?
✔✔To fix the crystal violet dye to the bacterial cell wall.
What is the counterstain used in the Gram staining process?
✔✔Safranin.
What is the purpose of heat-fixing a bacterial smear?
✔✔To kill the bacteria and adhere them to the slide.
1
,What does the term "aseptic technique" mean?
✔✔Methods used to prevent contamination of cultures and the environment.
What is the role of the condenser on a light microscope?
✔✔To focus light onto the specimen.
Which objective lens is typically used to observe bacteria on a light microscope?
✔✔The oil immersion lens (100x).
What does the term "pure culture" mean?
✔✔A culture containing only one type of microorganism.
What type of media is used to isolate and grow only specific types of bacteria?
✔✔Selective media.
Why is it important to incubate plates upside down?
✔✔To prevent condensation from dripping onto the agar surface.
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, What is the term for bacteria that grow in chains?
✔✔Streptococci.
What color do Gram-positive bacteria appear under the microscope?
✔✔Purple.
What is the purpose of the streak plate method?
✔✔To isolate individual colonies of bacteria.
What does the presence of bubbles during a catalase test indicate?
✔✔The production of oxygen from the breakdown of hydrogen peroxide.
What is the main purpose of using a broth culture in the lab?
✔✔To grow and maintain a larger volume of bacteria.
What is the shape of rod-shaped bacteria called?
✔✔Bacilli.
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