Lab 2 Culturing & Aseptic Technique BIO250L
Straighterline BIO250L Lab 2 Culturing & Aseptic Technique Completed Worksheet with all
lab photos (Latest Version August 2024)
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet.
Submissions without proper formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. This lab includes two experiments that each look at different aspects of culturing
microorganisms onto growth media. What concepts does each experiment focus on? Why do
you think these concepts are important to the field of microbiology?
These experiments focus on two core ideas in microbiology: bacterial inoculation and aseptic
method. In order to maintain the integrity of bacterial cultures, prevent contamination during
microbiological procedures, and guarantee correct results, aseptic technique is essential.
Growing and analyzing microbial populations requires the use of bacterial inoculation and
culture techniques, which isolate particular strains for in-depth investigation. For accurate
testing, microbial modification, and applications in environmental research, biotechnology, and
medicine, mastery of these fundamental ideas is essential.
2. In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools
and provide a scenario in which you would use each tool.
○ Microorganisms are transferred across plates and inoculum is collected from cultures
using inoculation loops.
○ Sterile cotton swabs are used to initiate cultures on plates and to gather microbiological
samples from different surfaces.
○ Inoculation needles are used to retrieve microorganisms from a specific area of a culture
and to inoculate semi-solid media or stab tubes..
○ Sterile plate spreaders are employed in the spread plate technique to inoculate plates,
ensuring an even distribution of microbial colonies.
3. Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes.
Focus particularly on those used with E. coli used to make human insulin.
An apparatus called a chemostat is used to maintain the growth pattern of lab cultures
throughout time. It continuously supplies fresh medium, an essential ingredient that encourages
bacterial growth and gets rid of cell waste.
4. You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to
diagnose a gastrointestinal infection. You streak your sample on a growth media containing
, Lab 2 Culturing & Aseptic Technique BIO250L
glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7. You
incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see
clear bacterial colonies forming on the plate. Would you feel confident in stating that you had
successfully cultured all the bacteria from your gut sample? Why or why not?
No, I wouldn't feel safe because the culture was only carried out in aerobic settings and
anaerobic situations were overlooked.
EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION
Introduction Questions
1. Describe the objectives of Experiment 1. State the goal of the experiment and the learning
outcome that it is trying to convey to you as a microbiology student.
Giving learners the practical experience they need for lab work is the aim of this experiment.
Teaching students the precise steps involved in setting up agar plates—such as measuring,
mixing, and sterilizing agar with nutrients—is the main goal. The experiment also intends to
teach students the correct methods for introducing bacteria into isolated colonies, either via
streaking or spreading. Students can examine and analyze bacterial growth patterns by
incubating bacterial cultures on agar plates. This will help them better understand how varied
incubation conditions affect the development of microorganisms.
2. Proper aseptic technique is crucial to ensuring the growth of pure bacterial colonies. What will
you do in this lab to demonstrate that you are using proper aseptic technique?
Aseptic procedures can be effectively ensured by minimizing the amount of time that media is
exposed to air when open.
In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
○ Dry heat sterilization
○ Autoclaving
○ Filter steralization
3. What method of sterilization will you use in this experiment?
autoclaving
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