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MCB5205 Exam 1 With Complete Solution
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MCB5205 Exam 1 WithComplete Solution
Pathogenesis - ANSWER the process in which a microbe causes a disease
pleomorphic theory of disease - ANSWER germs are the result of host
disease, not cause
Miasma Theory of Disease - ANSWER "poisonous" vapor from decomposing
materials causes disease.
lead to improved sanitation.
Germ Theory of Disease - ANSWER idea that infectious diseases are caused
by microorganisms
Koch's first postulate - ANSWER The same pathogen must be present in
every case of the disease
Koch's second postulate - ANSWER The pathogen must be isolated from the
diseased host and grown in pure culture
Koch's third postulate - ANSWER Microorganisms from the pure culture are
inoculated into a healthy, susceptible animal. The disease is reproduced.
Koch's fourth postulate - ANSWER The organism should be reisolated and
shown to be the same as the original
caveat of Koch's first postulate - ANSWER - send microbes only cause disease
in a small part of population.
, - some microbes are risk factors and not causes
caveat of Koch's second postulate - ANSWER - not all microbes can be
cultures (T. pallidum)
- not all diseases are caused by a single microbe
caveat of Koch's third postulate - ANSWER - finding a suitable host organism
Fifth postulate - ANSWER effective treatments that eliminate the organism
and disease can be used to further implicate a microbe as the cause of
disease
molecular Koch's postulate 1 - ANSWER Gene or it's product should only be
found in bacterial strains that cause disease
molecular Koch's postulates 2 - ANSWER Gene should be isolated by
molecular cloning
molecular Koch's postulates 3 - ANSWER mutations / disruption of the gene
should cause decreased virulence in vivo or introduction of the gene into and
a virulent strain should cause disease.
molecular Koch's postulates 4 - ANSWER Gene is expressed by bacteria in
vivo during infective process
caveats to molecular Koch's postulates - ANSWER -difficult for organisms
that have multiple virulence factors.
-genetic manipulation of some pathogens is difficult
- cannot be easily applied to polymicrobial/microbial shift diseases
molecular cloning - ANSWER isolation and incorporation of a piece of DNA
into a vector so it can be replicated and manipulated
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