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BIOCHEM 4300 EXAM WITH COMPLETE SOLUTIONS
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BIOCHEM 4300 EXAM WITH COMPLETE SOLUTIONS...
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BIOCHEM 4300 EXAM WITH COMPLETE SOLUTIONSFlicks first law - ANSWER A. Diffusion is driven by chemical potential
B.the rate of diffusion is proportional to the concentration gradient
C. Ficks first law law applies to steady state diffusion
Ficks first law cab be used to determine D (diffusion coefficient)
Diffusionis driven by chemical potential - ANSWER Molecules move from a region of high
concentration to a region of lower chemical potential
Ficks second law - ANSWER Second order partial differential equation. The solution is
strongly dependent on the system configuration
Ficks second law applies - ANSWER To non steady conditions eg concentration varies
with time
K (dehydration) G - ANSWER 203 kJ mol
Na (dehydration) G - ANSWER 301 kJ mol
Electrophoresis type - ANSWER Agarose and acrylamide
Electrophoresis - ANSWER Electrophoretic mobility is a result of a constant drift speed
Mobility of a macromolecule depends on its net charge, size and shape
Protein electrophoresis with PI (4.8) - ANSWER Ph=3, positive charge , migrates
towards -electrode
, Ph=4.8, neutral charge, no migration
Ph=7, negative charge, positive electrode
Sedimentation rate depends on - ANSWER Strength of the field on the masses and
shapes of the particles
It is very slow but can be accelerated by ultracentrifugation
Denatured proteins will - ANSWER Sediment fasted than folded globular protein
Surface matters due to - ANSWER Upward frictional force
Sedimentation by centrufugation - ANSWER 1 ) boundary sedimentation
2) zonal ( band ) sedimentation
Main purpose of boundary sedimentation by centrifufation is - ANSWER Not just to
separate , using it to analyze pure species and hopefully get MW a info
Boundary sedminentation - ANSWER Employs and analytical ultra centrifuge
Initially the solute is uniformly distributed throughout the sample cell
During centriguation the solute moves down the sample column producing a boundary
between pure solvent and the solution
Zonal band sedimentation - ANSWER Uses a conventional ultracentrifuge
Sample is layered over a density gradient ( produced with sucrose or glycerol)
At the end of the run the contents of tubes are fractions and analyzed
Sedimentation velocity - ANSWER A solute molecule of mass m being spun in a
centrifuge experience three forces
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