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Moleculaire biologie en DNA-technologie samenvatting H6 en H10
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Bevat samenvatting van: - regulatie genexpressie - technologie Geslaagd met 15/20
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Moleculaire biologie en DNA-technologie:Samenvatting H6 en 10
, Hoofdstuk 6 :
Regulatie genexpressie
constitutieve
genexpressie aangemaakt ubactine
worolt steeds vereist dus voordurend ,
Geïnduceerde
genexpressie is enkel vereist in bepaalde omstandigheden is reactie op , een
externe
signalen vb lactose
bijprokamyoten
insuline aanmaak
bij erkaryoten als
glucose aanwezig is
1
Genexpressie prokaryoot vs
erkaryoof
vaak
polycistronisch
1.1 -
mRNA codeert
: meerdere eiwitten
voor
-verschillende open lees ramen met stopcodon
eigen start- ,
en
initiator AUG
Shine-Dalgarno upstream van
-
1 2
.
- mature
eukaryotische mRNA meestal monocistronisch
initiatie meestal
op eerste AUG in MRNA
-
2
Regulatie genexpressie prokaryoten in
OPERON functioneel samenhoren vertaald worden
2 1 .
: set van
genen
die , samen worden
overgeschreven naar mRNA en samen
naar eiwitten. De
volledige keten staat onder gemeenschappelijke controle .
Promotor :
Signaal RNA polymerase te
beginnenom
transcriptie aan
Operator :
plaats waar repressor bindt
2 2 .
lac operon
Lactose is dissacharide en wordt
omgezet in galactose en glucose .
Lac operon bevat alle
enzymen noolig voor de afbraak van lactose
.
-
galactosiole permease
-
beta-galactosiolase
-
transacetylase
Geïnduceerde
genexpressie -=
negatieve regulatie :
repressor
Lactose bindt aan actieve represeor : inactieve Repressor
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