Microbiology Lab Exam 1 Questions and Answers Already Passed
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Course
Microbiology Lab
Institution
Microbiology Lab
Microbiology Lab Exam 1 Questions and
Answers Already Passed
What color indicates a positive urease test?
Bright pink or fuchsia.
What is the purpose of the citrate test?
To determine if bacteria can use citrate as their sole carbon source.
What color change indicates a positive cit...
Microbiology Lab Exam 1 Questions and
Answers Already Passed
What color indicates a positive urease test?
✔✔ Bright pink or fuchsia.
What is the purpose of the citrate test?
✔✔ To determine if bacteria can use citrate as their sole carbon source.
What color change indicates a positive citrate test?
✔✔ Blue.
What is the purpose of serial dilution in microbiology?
✔✔ To reduce bacterial concentration for counting colonies.
What is the role of the autoclave in a microbiology lab?
✔✔ To sterilize equipment and media using high-pressure steam.
1
,What does a spectrophotometer measure in microbiology?
✔✔ The turbidity or optical density of a bacterial culture.
What is the purpose of a control in an experiment?
✔✔ To provide a baseline for comparison and validate results.
What are biofilms in microbiology?
✔✔ Communities of microorganisms attached to a surface and embedded in a matrix.
What is the ecological role of decomposer bacteria?
✔✔ To break down organic matter and recycle nutrients.
What is the purpose of using a flame in aseptic techniques?
✔✔ To sterilize tools and create a sterile environment by reducing airborne contaminants.
What is the importance of aseptic technique in a microbiology lab?
✔✔ Aseptic technique prevents contamination of cultures and the environment.
2
,What is the purpose of a streak plate method?
✔✔ To isolate individual bacterial colonies for pure culture.
What is a colony in microbiology?
✔✔ A visible cluster of bacteria derived from a single cell growing on solid media.
What type of microscope is most commonly used in microbiology labs?
✔✔ A compound light microscope.
What is the magnification of an objective lens labeled 40x when used with a 10x ocular lens?
✔✔ 400x.
What is the purpose of using immersion oil with a microscope?
✔✔ To improve resolution by reducing light refraction.
What type of dye is used in a simple stain?
✔✔ Basic dyes like methylene blue or crystal violet.
3
, What is the purpose of heat-fixing a bacterial smear?
✔✔ To kill the bacteria and adhere them to the slide.
What does Gram staining differentiate?
✔✔ It differentiates bacteria based on their cell wall structure into Gram-positive and Gram-
negative.
What is the primary stain in Gram staining?
✔✔ Crystal violet.
What is the counterstain in the Gram stain procedure?
✔✔ Safranin.
What color do Gram-positive bacteria appear after Gram staining?
✔✔ Purple.
What color do Gram-negative bacteria appear after Gram staining?
✔✔ Pink or red.
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