Microbiology Lab Quiz 1 Latest Version
Graded A+
What is a pure culture?
A culture containing only one species of microorganism.
What is aseptic technique used for?
To prevent contamination of samples, equipment, and the environment.
What does a colony on an agar plate represent?
...
Microbiology Lab Quiz 1 Latest Version
Graded A+
What is a pure culture?
✔✔ A culture containing only one species of microorganism.
What is aseptic technique used for?
✔✔ To prevent contamination of samples, equipment, and the environment.
What does a colony on an agar plate represent?
✔✔ A group of cells originating from a single parent cell.
What is the role of agar in microbiological media?
✔✔ To provide a solid surface for microbial growth.
What temperature is commonly used to incubate bacteria in the lab?
✔✔ 37°C, which is close to human body temperature.
1
,What is the term for bacteria that grow best at moderate temperatures?
✔✔ Mesophiles.
What is the purpose of a streak plate technique?
✔✔ To isolate individual bacterial colonies from a mixed culture.
Why is it important to flame the inoculating loop before and after use?
✔✔ To sterilize the loop and prevent contamination.
What does turbidity in a liquid culture indicate?
✔✔ Bacterial growth.
What is microbiology the study of?
✔✔ The study of microorganisms, including bacteria, viruses, fungi, protozoa, and algae.
What type of microscope is commonly used to observe bacteria?
✔✔ A compound light microscope.
2
, What is the purpose of immersion oil in microscopy?
✔✔ To increase the resolution by reducing light refraction.
What is the magnification of the ocular lens in most compound microscopes?
✔✔ 10x.
What is the total magnification when using a 40x objective lens and a 10x ocular lens?
✔✔ 400x.
What is the primary purpose of a simple stain?
✔✔ To enhance contrast and make the morphology of cells visible.
What type of dye is used in a simple staining procedure?
✔✔ Basic dye such as methylene blue or crystal violet.
What is the primary difference between prokaryotic and eukaryotic cells?
✔✔ Prokaryotic cells lack a nucleus, while eukaryotic cells have a nucleus.
3
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