Microbiology Lab: Written Exam 1 Questions and Answers Rated A+
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Microbiology Lab: Written
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Microbiology Lab: Written
Microbiology Lab: Written Exam 1
Questions and Answers Rated A+
What is the function of the ocular lens in a microscope?
To magnify the image formed by the objective lens.
Why is aseptic technique important in a microbiology lab?
To prevent contamination of the culture and the environmen...
Microbiology Lab: Written Exam 1
Questions and Answers Rated A+
What is the function of the ocular lens in a microscope?
✔✔ To magnify the image formed by the objective lens.
Why is aseptic technique important in a microbiology lab?
✔✔ To prevent contamination of the culture and the environment.
What is the role of agar in culture media?
✔✔ It provides a solid surface for microbial growth.
Why is immersion oil used with a 100x objective lens?
✔✔ To reduce light refraction and improve resolution.
What is the primary use of a streak plate technique?
✔✔ To isolate individual colonies from a mixed bacterial culture.
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,What is the purpose of heat-fixing a bacterial smear before staining?
✔✔ To kill the bacteria and fix them to the slide.
What is a pure culture in microbiology?
✔✔ A culture containing only one species of microorganism.
What type of microorganism is commonly used as a model organism in microbiology?
✔✔ Escherichia coli (E. coli).
What is the main difference between eukaryotic and prokaryotic cells?
✔✔ Eukaryotic cells have a nucleus, while prokaryotic cells do not.
What does the term "colony morphology" refer to?
✔✔ The physical characteristics of microbial colonies, such as size, shape, and color.
What is the purpose of a selective medium?
✔✔ To allow the growth of specific microorganisms while inhibiting others.
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, What is an autoclave used for in microbiology labs?
✔✔ To sterilize equipment and media using high-pressure steam.
What is the significance of endospores in bacteria?
✔✔ They allow bacteria to survive harsh environmental conditions.
How does a Gram stain differentiate between bacterial types?
✔✔ It distinguishes bacteria based on the composition of their cell walls, with Gram-positive
retaining the crystal violet stain and Gram-negative appearing pink after counterstaining
How can you differentiate motile bacteria from non-motile bacteria in the lab?
✔✔ By using a motility test, such as hanging drop or semi-solid agar.
What is the purpose of a negative stain?
✔✔ To visualize structures that are difficult to stain, such as capsules, by staining the
background.
Why is it necessary to incubate plates upside down?
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