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Microbiology Lab Practical 1 (Pearson Education Edition) Questions and Answers 100% Pass
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Microbiology Lab Practical 1 (Pearson Education Edition) Questions and Answers 100% Pass What is the purpose of staining bacterial cells? To increase contrast and make the cells visible under a microscope. What is the primary dye used in Gram staining? Crystal violet. Why is iodine...

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  • December 22, 2024
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Microbiology Lab Practical 1 (Pearson
Education Edition) Questions and
Answers 100% Pass
What is the purpose of staining bacterial cells?


✔✔ To increase contrast and make the cells visible under a microscope.




What is the primary dye used in Gram staining?


✔✔ Crystal violet.




Why is iodine used in Gram staining?


✔✔ It acts as a mordant to form a complex with the crystal violet, making it adhere better to the

bacterial cell walls.




What color do Gram-positive bacteria appear after staining?


✔✔ Purple.




What color do Gram-negative bacteria appear after staining?


✔✔ Pink or red.


1

,Why is heat fixation performed before staining a bacterial smear?


✔✔ To kill the bacteria and fix them to the slide.




What is the main difference between Gram-positive and Gram-negative bacterial cell walls?


✔✔ Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have

a thin peptidoglycan layer and an outer membrane.




What is the purpose of using safranin in the Gram stain procedure?


✔✔ It is a counterstain that colors Gram-negative bacteria pink.




What is the shape of cocci bacteria?


✔✔ Spherical.




What is the shape of bacilli bacteria?


✔✔ Rod-shaped.




What is the function of the condenser on a microscope?


2

,✔✔ To focus light onto the specimen.




Why is oil immersion used with the 100x objective lens?


✔✔ To reduce light refraction and increase resolution.




What is the purpose of the aseptic technique in microbiology?


✔✔ To prevent contamination of cultures and the environment.




What is the proper way to sterilize an inoculating loop?


✔✔ By holding it in the flame of a Bunsen burner until it glows red.




What is the function of the decolorizer in Gram staining?


✔✔ To remove the primary stain from Gram-negative cells.




What is the typical magnification achieved with a compound light microscope?


✔✔ Up to 1000x.




Why is it important to label agar plates on the bottom rather than the lid?

3

, ✔✔ To ensure the label remains with the sample even if the lid is misplaced.




What is a pure culture?


✔✔ A culture containing only one type of microorganism.




What is the significance of the zone of inhibition in an antibiotic susceptibility test?


✔✔ It indicates the effectiveness of the antibiotic against the tested microorganism.




What is the purpose of streaking for isolation on an agar plate?


✔✔ To obtain isolated colonies of bacteria.




How can you differentiate between a bacterial colony and a fungal colony on an agar plate?


✔✔ Bacterial colonies are usually smooth and shiny, while fungal colonies are often fuzzy or

powdery.




What is the purpose of using a selective medium in microbiology?


✔✔ To allow the growth of specific microorganisms while inhibiting others.




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