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Selfstudy assignment answers lecture 14-15
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1. What is a bladder cystoplasty?Any plactic or reconstructive operation on the bladder , for example an bladder
augmentation (enlargement).
2. What materials were used for the scaffold? Which material performed best?
collagen, or a composite of collagen and polyglycolic acid. The collagen-polyglycolic acid
scaffold worked the best
3. What method of sterilization was applied?
Ethylene oxide gas sterilization to sterilize the collagen-based scaffolds used for bladder
regeneration. Ethylene oxide is a common method for sterilizing medical devices and
biological materials, especially when heat-sensitive materials are involved, as it can
effectively sterilize without damaging the scaffold's structure. They combined UV light with
ethylene oxide
Other types of sterilization
- Heat treatment (steaming), protein get denatured at a high temperature so the
scaffold will be degraded or damage quite a bit
- Gamma sterilization, this will damage or degrade the scaffold
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4. What cells were used for seeding? What are (dis)advantages of the use of autologous
cells?
Urothelial and muscle cells. The use of autologous cells is very time consuming and the
health of the cells can be affected by factors such as age, disease or previous treatments.
Advantages or using autologous cells?
- No immune response, since patients own cells are used
Disadvantage
- Invasive to get the cells
- You need to do the treatment for each person so it takes more time
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