Biotech Final Exam Questions with
Complete Solutions (Latest 2025) 115 Qs
The manipulation of cellular function by DNA modification
(T/F) Srid - Correct Answers ✅Genetic Engineering
Deoxyribose + sugar = ? Srid
What's a Nucleotide?
What's a Nucleoside? - Correct Answers ✅DNA nucleoside
- Phosphate + sugar + base
- Sugar + base
What does messenger RNA (mRNA) do? Srid - Correct
Answers ✅mRNA guides protein synthesis by using the DNA
segment information
RNA is single-stranded
Central Dogma of Molecular Biology Srid - Correct Answers
✅DNA → transcription → RNA → translation → protein
Proteins are mediators of biological function
The complete haploid genetic complement of an organism is?
(DEF) Srid - Correct Answers ✅Genome
,Biotech Final Exam Questions with
Complete Solutions (Latest 2025) 115 Qs
The study of DNA on a cellular scale is? (DEF) Srid - Correct
Answers ✅Genomics
What are Exons? Srid
What are Introns? - Correct Answers ✅Coding segments of
DNA
Non-translated DNA
Selective amplification of a particular gene or DNA segment
so that its genetic information may be studied and utilized
(T/F) Srid
The methods used to accomplish DNA cloning and related
task (T/F) - Correct Answers ✅DNA cloning
Recombinant DNA technology or Genetic engineering
Small DNAs capable of autonomous replication Srid - Correct
Answers ✅Cloning vectors
Advantages of cloning in E.coli Srid - Correct Answers ✅1.
DNA metabolism is well understood
2. Many naturally occurring cloning vectors (plasmids and
bacteriophages)
3. Techniques for moving DNA from one bacterial cell to
another
, Biotech Final Exam Questions with
Complete Solutions (Latest 2025) 115 Qs
What joins the DNA fragment to be cloned to a suitable
cloning vector? Srid
- recognize and cleave DNA at specific sequences
- catalyze methylation of host DNA (protect from RE)
- the restriction endonuclease and the corresponding
methylase
RE can cut this gene...? No? Srid
RE leave stick or blunt ends on DNA from the Palindrome
sequence
Presence of unpaired bases on the ends
there are no unpaired bases on the ends - Correct Answers
✅Sticky ends
Blunt ends
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