produced against specific toxin eg.spider
bites.
results in rapid cure
introduction
eg. the Serum Run during Diphtheria
outbreak in 1925.
1. A Small dose of toxin is injected into an
animal, e.g. Horse or Goat.
2. It makes antibodies against toxin
antiserum
3. Blood collected, and “serum” purified
production
4. Antibody isolated
5. used in injection
very difficult to get pure antibody
animal antibodies differ form human
problems
difficult, expensive and unethical
possibility of allergy
a solution to these problems invented in
1975 by Milstein and Kohler who won Pas
Nobel Prize
1. antigen X are injected into a mouse to
stimulate its production of antibody
against X
2. B lymphocyte are then isolated from
mouse's blood
these cells are fused to cancer cells (
production
myeloma) which are immortal to ensure
bites.
results in rapid cure
introduction
eg. the Serum Run during Diphtheria
outbreak in 1925.
1. A Small dose of toxin is injected into an
animal, e.g. Horse or Goat.
2. It makes antibodies against toxin
antiserum
3. Blood collected, and “serum” purified
production
4. Antibody isolated
5. used in injection
very difficult to get pure antibody
animal antibodies differ form human
problems
difficult, expensive and unethical
possibility of allergy
a solution to these problems invented in
1975 by Milstein and Kohler who won Pas
Nobel Prize
1. antigen X are injected into a mouse to
stimulate its production of antibody
against X
2. B lymphocyte are then isolated from
mouse's blood
these cells are fused to cancer cells (
production
myeloma) which are immortal to ensure
