NMTCB PET CERTIFICATION
REVIEW QUESTIONS |
GUARANTEED ACCURATE
ANSWERS
Rb-82 Genertaor Alert Level - ACCURATE ANSWERS✔✔ 14L of
eleute volume reached, or Sr-82 level 0.002uCi/mCi of Rb-82, or Sr-85
level 0.02uCi/mCi of Rb-82
Actions for Rb82 Generator Alert Level - ACCURATE ANSWERS✔✔
Test after every 750 mL has passed through generator
Stop using Rb-82 generator if - ACCURATE ANSWERS✔✔ 17L of
eleute volume reached, or 42 days post calibration date, or Sr82 levenis
0.01uCi/mCi of Rb82, or Sr85 level reaches 0.1uCi/mCi of Rb82
Cyclotron produced isotopes and half-life - ACCURATE
ANSWERS✔✔ C11, 20.3 min; N13 9.97 min; O15 2.03 min; F18,
109.7 min
Advantages of negative ion cyclotrons - ACCURATE ANSWERS✔✔ 1)
less nuclear activation of cyl potion cavity and surroundings, 2) can self-
focus beam of particles, 3) can irradiate 2 targets at once
,Isotopes produces by negative ion cyclotrons - ACCURATE
ANSWERS✔✔ C, N, O = metabolites
Reaction for chemical labeling of F18-FDG - ACCURATE
ANSWERS✔✔ Nucleophilic substitution
Chemical reaction of FDG - ACCURATE ANSWERS✔✔ Electron rich
group of atoms change place on molecule with a positively charged
leaving group
Precursor molecule for FDG - ACCURATE ANSWERS✔✔ Mannose
triflate
Chem formula of FDG - ACCURATE ANSWERS✔✔ 2-deoxy-2-
[18F]fluoro-D-glucose. (Fludeoxyglucose F-18)
Intermediate step in FDG synthesis - ACCURATE ANSWERS✔✔
Alkaline hydrolysis of 4 acetyl groups by replacing with OH
Size filter used after purification - ACCURATE ANSWERS✔✔ 0.22 um
FDA ref for PET pharma production - ACCURATE ANSWERS✔✔
General chapter 823
,FDG QA appearance - ACCURATE ANSWERS✔✔ Colorless liquid
FDG QA radiochemical identity - ACCURATE ANSWERS✔✔ Thin-
layer chromatography Rf value to reference standard
FDG QARadionuclide identity - ACCURATE ANSWERS✔✔ Half-life
105 and 115 minutes
FDG QA bacterial endotoxins test - ACCURATE ANSWERS✔✔ LAL
(Limulus amebocyte lysate) <175 USP endotoxins units per max volume
injection; endotoxins< 17.5 EU
FDG QC pH - ACCURATE ANSWERS✔✔ Between 4.5 to 7.5
FDG QA Radiochemical purity - ACCURATE ANSWERS✔✔ Thin-
layered chromatography radioactive Rf should be not less than 90% of
total radioactivity of chromatography plate
FDG QC Radionuclideic purity - ACCURATE ANSWERS✔✔ should
be >99.5% of radionuclidie specified. tested using multi-channel
analyzer with peaks at 0.511 and 1.022 MeV
FDG QC chemical purity - ACCURATE ANSWERS✔✔ testing
depends upon synthesis method used to create FDG
, Kryptofix 222 - ACCURATE ANSWERS✔✔ FDG QC chemical purity
test - thin-layer paper chromatography spot test. 50 ug/mL and FDG
solution must demonstrate a less intense response than standard
2-Chloro-2-deoxy-D-glucose - ACCURATE ANSWERS✔✔ FDG QC
chemical purity test used if hydrolysis step was performed with
hydrochloric acid. Use high-performance liquid chromatography using
an electrochemical detector. Mus not have more than 1 mg CLDG: 2-
deoxy-2-D-glucose
FDG QC Residual solvents - ACCURATE ANSWERS✔✔ solvents can
be in FDG from cleaning or synthesis solutions. solutions may contain
acetonitrle, ether anddehydrated alcohol. no more 0.04% acetonitrle, and
less than 0.5% of ether or ethanol
FDG QC Sterility - ACCURATE ANSWERS✔✔ not performed before
dosing due to need of 14 days to perform. Regulations require final
produce to be placed in soy broth mixture with a thioglycollate growth
media for 30 hrs. look for observable bacteria
FDG QC Filter membrane integerity - ACCURATE ANSWERS✔✔
final sterility filter used at end of manufacturing process. Filter must
remain intact as pressure increased to 50 psi (bubble point test)
REVIEW QUESTIONS |
GUARANTEED ACCURATE
ANSWERS
Rb-82 Genertaor Alert Level - ACCURATE ANSWERS✔✔ 14L of
eleute volume reached, or Sr-82 level 0.002uCi/mCi of Rb-82, or Sr-85
level 0.02uCi/mCi of Rb-82
Actions for Rb82 Generator Alert Level - ACCURATE ANSWERS✔✔
Test after every 750 mL has passed through generator
Stop using Rb-82 generator if - ACCURATE ANSWERS✔✔ 17L of
eleute volume reached, or 42 days post calibration date, or Sr82 levenis
0.01uCi/mCi of Rb82, or Sr85 level reaches 0.1uCi/mCi of Rb82
Cyclotron produced isotopes and half-life - ACCURATE
ANSWERS✔✔ C11, 20.3 min; N13 9.97 min; O15 2.03 min; F18,
109.7 min
Advantages of negative ion cyclotrons - ACCURATE ANSWERS✔✔ 1)
less nuclear activation of cyl potion cavity and surroundings, 2) can self-
focus beam of particles, 3) can irradiate 2 targets at once
,Isotopes produces by negative ion cyclotrons - ACCURATE
ANSWERS✔✔ C, N, O = metabolites
Reaction for chemical labeling of F18-FDG - ACCURATE
ANSWERS✔✔ Nucleophilic substitution
Chemical reaction of FDG - ACCURATE ANSWERS✔✔ Electron rich
group of atoms change place on molecule with a positively charged
leaving group
Precursor molecule for FDG - ACCURATE ANSWERS✔✔ Mannose
triflate
Chem formula of FDG - ACCURATE ANSWERS✔✔ 2-deoxy-2-
[18F]fluoro-D-glucose. (Fludeoxyglucose F-18)
Intermediate step in FDG synthesis - ACCURATE ANSWERS✔✔
Alkaline hydrolysis of 4 acetyl groups by replacing with OH
Size filter used after purification - ACCURATE ANSWERS✔✔ 0.22 um
FDA ref for PET pharma production - ACCURATE ANSWERS✔✔
General chapter 823
,FDG QA appearance - ACCURATE ANSWERS✔✔ Colorless liquid
FDG QA radiochemical identity - ACCURATE ANSWERS✔✔ Thin-
layer chromatography Rf value to reference standard
FDG QARadionuclide identity - ACCURATE ANSWERS✔✔ Half-life
105 and 115 minutes
FDG QA bacterial endotoxins test - ACCURATE ANSWERS✔✔ LAL
(Limulus amebocyte lysate) <175 USP endotoxins units per max volume
injection; endotoxins< 17.5 EU
FDG QC pH - ACCURATE ANSWERS✔✔ Between 4.5 to 7.5
FDG QA Radiochemical purity - ACCURATE ANSWERS✔✔ Thin-
layered chromatography radioactive Rf should be not less than 90% of
total radioactivity of chromatography plate
FDG QC Radionuclideic purity - ACCURATE ANSWERS✔✔ should
be >99.5% of radionuclidie specified. tested using multi-channel
analyzer with peaks at 0.511 and 1.022 MeV
FDG QC chemical purity - ACCURATE ANSWERS✔✔ testing
depends upon synthesis method used to create FDG
, Kryptofix 222 - ACCURATE ANSWERS✔✔ FDG QC chemical purity
test - thin-layer paper chromatography spot test. 50 ug/mL and FDG
solution must demonstrate a less intense response than standard
2-Chloro-2-deoxy-D-glucose - ACCURATE ANSWERS✔✔ FDG QC
chemical purity test used if hydrolysis step was performed with
hydrochloric acid. Use high-performance liquid chromatography using
an electrochemical detector. Mus not have more than 1 mg CLDG: 2-
deoxy-2-D-glucose
FDG QC Residual solvents - ACCURATE ANSWERS✔✔ solvents can
be in FDG from cleaning or synthesis solutions. solutions may contain
acetonitrle, ether anddehydrated alcohol. no more 0.04% acetonitrle, and
less than 0.5% of ether or ethanol
FDG QC Sterility - ACCURATE ANSWERS✔✔ not performed before
dosing due to need of 14 days to perform. Regulations require final
produce to be placed in soy broth mixture with a thioglycollate growth
media for 30 hrs. look for observable bacteria
FDG QC Filter membrane integerity - ACCURATE ANSWERS✔✔
final sterility filter used at end of manufacturing process. Filter must
remain intact as pressure increased to 50 psi (bubble point test)
