Week 2 - BIOS 242 EXAM QUESTIONS
WITH CORRECT ANSWERS
purpose |of |staining |- |VERIFIED |ANSWER✔✔-differentiating |between |different |cells |
(looks |at |internal |organelles, |flagella, |cell |walls, |how |it |moves, |spores)
metric |system |- |VERIFIED |ANSWER✔✔-can |measure |a |smaller |amount |(standardized
|- |meaning |its |easy |to |go |up |and |down)
microscopy |- |VERIFIED |ANSWER✔✔-the |use |of |light |or |electrons |to |magnify |objects
Antoni |van |Leeuwenhoek |- |VERIFIED |ANSWER✔✔-Who |used |the |first |microscope?
wavelength |- |VERIFIED |ANSWER✔✔-the |distance |between |two |corresponding |parts |
of |a |wave
magnification |- |VERIFIED |ANSWER✔✔-an |apparent |increase |in |the |size |of |an |object
resolution |- |VERIFIED |ANSWER✔✔-the |ability |to |distinguish |two |points |that |are |close |
to |one |another |(the |better |it |is, |the |better |you |can |distinguish |two |points)
contrast |- |VERIFIED |ANSWER✔✔-differences |in |intensity |between |two |objects |or |
between |an |object |and |its |background
contrast |between |what |we |are |looking |at |and |the |objects |surrounding |it |- |VERIFIED |
ANSWER✔✔-staining |increases |what?
scanning |tunnel |microscope |- |VERIFIED |ANSWER✔✔-needed |for |the |smallest |things
simple |microscopes |- |VERIFIED |ANSWER✔✔-contain |a |single |magnifying |lens |- |
similar |to |a |magnifying |glass
bright-field |microscopes |- |VERIFIED |ANSWER✔✔-means |background |is |bright
compound |microscope |- |VERIFIED |ANSWER✔✔-series |of |lenses |for |magnification, |
light |passes |through |specimen |into |objective |lens, |and |most |have |a |condenser |lens
compound |- |VERIFIED |ANSWER✔✔-what |kind |of |microscope |do |we |use |in |class
oil |immersion |lens |- |VERIFIED |ANSWER✔✔-increases |resolution |by |changing |angle |of
|refraction |(doesn't |allow |light |to |escape |from |sides)
total |magnification |- |VERIFIED |ANSWER✔✔-magnification |of |objective |lens |x |
magnification |of |ocular |lens
ocular |lens |- |VERIFIED |ANSWER✔✔-remagnifies |the |image |formed |by |the |objective |
lens
body |- |VERIFIED |ANSWER✔✔-transmits |the |image |from |the |objective |lens |to |the |
ocular |lens |using |prisms
objective |lens |- |VERIFIED |ANSWER✔✔-primary |lens |that |magnify |the |specimen |
(usually |3)
, stage |- |VERIFIED |ANSWER✔✔-holds |the |micrscope |slide |in |position
condenser |- |VERIFIED |ANSWER✔✔-focuses |light |through |specimen
diaphragm |- |VERIFIED |ANSWER✔✔-controls |the |amount |of |light |entering |the |
condenser
illuminator |- |VERIFIED |ANSWER✔✔-light |source
coarse |focusing |knob |- |VERIFIED |ANSWER✔✔-moves |the |stage |up |and |down |to |focus |
the |image
fine |focusing |knob |- |VERIFIED |ANSWER✔✔-Slightly |moves |the |stage |up |and |down |for |
focusing
dark |field |microscopes |- |VERIFIED |ANSWER✔✔-background |is |dark, |best |for |
observing |pale |objects |because |they |will |appear |light |against |the |dark |background, |
increases |detail
phase |microscopes |- |VERIFIED |ANSWER✔✔-Used |to |examine |living |organisms |or |
specimens |that |would |be |damaged/altered |by |attaching |them |to |slides |or |staining
phase-contrast |microscopes |- |VERIFIED |ANSWER✔✔-produce |sharply |defined |images
|in |which |fine |structures |can |be |seen |in |living |cells
differential |interference |contrast |microscopes |- |VERIFIED |ANSWER✔✔-significantly |
increase |contrast |and |give |an |image |a |dramatic |3D |and |shadowed |appearance
fluorescence |microscpes |- |VERIFIED |ANSWER✔✔-takes |advantage |of |stains |by |using |
a |direct |UV |light |source |at |specimen |which |increases |resolution |and |contrast
immunofluorescence |- |VERIFIED |ANSWER✔✔-means |they |use |antibodies |to |take |the |
stain |to |the |organism |(antibodies |can |only |bind |to |certain |things)
confocal |microscope |- |VERIFIED |ANSWER✔✔-uses |fluorescent |dyes, |uses |UV |lasers |
to |illuminate |fluorescent |chemicals |in |a |single |plane, |and |resolution |is |increased |
because |emitted |light |passes |through |pinhole |aperture
confocal |- |VERIFIED |ANSWER✔✔-what |microscope |do |you |use |to |look |at |biofilms?
electron |microscope |- |VERIFIED |ANSWER✔✔-cannot |resolve |structures |smaller |than |
200 |nm, |have |greater |resolving |power |and |magnification, |and |provides |detailed |views |
of |bacteria, |viruses, |internal |cellular |structures, |molecules, |and |large |atoms
electron |- |VERIFIED |ANSWER✔✔-which |microscope |sends |light |from |top |to |bottom?
electron |- |VERIFIED |ANSWER✔✔-which |microscope |must |the |object |be |dead |because |
of |a |vacuum?
electron |- |VERIFIED |ANSWER✔✔-what |microscope |produces |all |black |and |white |
images?
probe |microscope |- |VERIFIED |ANSWER✔✔-magnifies |more |than |100 |million |times
WITH CORRECT ANSWERS
purpose |of |staining |- |VERIFIED |ANSWER✔✔-differentiating |between |different |cells |
(looks |at |internal |organelles, |flagella, |cell |walls, |how |it |moves, |spores)
metric |system |- |VERIFIED |ANSWER✔✔-can |measure |a |smaller |amount |(standardized
|- |meaning |its |easy |to |go |up |and |down)
microscopy |- |VERIFIED |ANSWER✔✔-the |use |of |light |or |electrons |to |magnify |objects
Antoni |van |Leeuwenhoek |- |VERIFIED |ANSWER✔✔-Who |used |the |first |microscope?
wavelength |- |VERIFIED |ANSWER✔✔-the |distance |between |two |corresponding |parts |
of |a |wave
magnification |- |VERIFIED |ANSWER✔✔-an |apparent |increase |in |the |size |of |an |object
resolution |- |VERIFIED |ANSWER✔✔-the |ability |to |distinguish |two |points |that |are |close |
to |one |another |(the |better |it |is, |the |better |you |can |distinguish |two |points)
contrast |- |VERIFIED |ANSWER✔✔-differences |in |intensity |between |two |objects |or |
between |an |object |and |its |background
contrast |between |what |we |are |looking |at |and |the |objects |surrounding |it |- |VERIFIED |
ANSWER✔✔-staining |increases |what?
scanning |tunnel |microscope |- |VERIFIED |ANSWER✔✔-needed |for |the |smallest |things
simple |microscopes |- |VERIFIED |ANSWER✔✔-contain |a |single |magnifying |lens |- |
similar |to |a |magnifying |glass
bright-field |microscopes |- |VERIFIED |ANSWER✔✔-means |background |is |bright
compound |microscope |- |VERIFIED |ANSWER✔✔-series |of |lenses |for |magnification, |
light |passes |through |specimen |into |objective |lens, |and |most |have |a |condenser |lens
compound |- |VERIFIED |ANSWER✔✔-what |kind |of |microscope |do |we |use |in |class
oil |immersion |lens |- |VERIFIED |ANSWER✔✔-increases |resolution |by |changing |angle |of
|refraction |(doesn't |allow |light |to |escape |from |sides)
total |magnification |- |VERIFIED |ANSWER✔✔-magnification |of |objective |lens |x |
magnification |of |ocular |lens
ocular |lens |- |VERIFIED |ANSWER✔✔-remagnifies |the |image |formed |by |the |objective |
lens
body |- |VERIFIED |ANSWER✔✔-transmits |the |image |from |the |objective |lens |to |the |
ocular |lens |using |prisms
objective |lens |- |VERIFIED |ANSWER✔✔-primary |lens |that |magnify |the |specimen |
(usually |3)
, stage |- |VERIFIED |ANSWER✔✔-holds |the |micrscope |slide |in |position
condenser |- |VERIFIED |ANSWER✔✔-focuses |light |through |specimen
diaphragm |- |VERIFIED |ANSWER✔✔-controls |the |amount |of |light |entering |the |
condenser
illuminator |- |VERIFIED |ANSWER✔✔-light |source
coarse |focusing |knob |- |VERIFIED |ANSWER✔✔-moves |the |stage |up |and |down |to |focus |
the |image
fine |focusing |knob |- |VERIFIED |ANSWER✔✔-Slightly |moves |the |stage |up |and |down |for |
focusing
dark |field |microscopes |- |VERIFIED |ANSWER✔✔-background |is |dark, |best |for |
observing |pale |objects |because |they |will |appear |light |against |the |dark |background, |
increases |detail
phase |microscopes |- |VERIFIED |ANSWER✔✔-Used |to |examine |living |organisms |or |
specimens |that |would |be |damaged/altered |by |attaching |them |to |slides |or |staining
phase-contrast |microscopes |- |VERIFIED |ANSWER✔✔-produce |sharply |defined |images
|in |which |fine |structures |can |be |seen |in |living |cells
differential |interference |contrast |microscopes |- |VERIFIED |ANSWER✔✔-significantly |
increase |contrast |and |give |an |image |a |dramatic |3D |and |shadowed |appearance
fluorescence |microscpes |- |VERIFIED |ANSWER✔✔-takes |advantage |of |stains |by |using |
a |direct |UV |light |source |at |specimen |which |increases |resolution |and |contrast
immunofluorescence |- |VERIFIED |ANSWER✔✔-means |they |use |antibodies |to |take |the |
stain |to |the |organism |(antibodies |can |only |bind |to |certain |things)
confocal |microscope |- |VERIFIED |ANSWER✔✔-uses |fluorescent |dyes, |uses |UV |lasers |
to |illuminate |fluorescent |chemicals |in |a |single |plane, |and |resolution |is |increased |
because |emitted |light |passes |through |pinhole |aperture
confocal |- |VERIFIED |ANSWER✔✔-what |microscope |do |you |use |to |look |at |biofilms?
electron |microscope |- |VERIFIED |ANSWER✔✔-cannot |resolve |structures |smaller |than |
200 |nm, |have |greater |resolving |power |and |magnification, |and |provides |detailed |views |
of |bacteria, |viruses, |internal |cellular |structures, |molecules, |and |large |atoms
electron |- |VERIFIED |ANSWER✔✔-which |microscope |sends |light |from |top |to |bottom?
electron |- |VERIFIED |ANSWER✔✔-which |microscope |must |the |object |be |dead |because |
of |a |vacuum?
electron |- |VERIFIED |ANSWER✔✔-what |microscope |produces |all |black |and |white |
images?
probe |microscope |- |VERIFIED |ANSWER✔✔-magnifies |more |than |100 |million |times
