Summary
Summary HC.7 - electron microscopy
- Course
- Institution
Summary HC.7 Electron Microscopy - Course: Light and Electron Microscopy UU
[Show more]
Seller
Follow
annickkooij
Reviews received
Content preview
Samenvatting EM HC.7Correlative light and electron microscopy (CLEM)
Licht
microscopy:
positional
and dynamic
information
on specific
biomolecules
Electron microscopy: high-resolution information
on cellular ultrastructure and protein structure
When you want to image as close to native conditions, you need to image in cryo. There are
only a few challenges:
The objective lens in LM need to be functional in liquid nitrogen temperatures. When this
is not the case, you can work with air objectives: not fully immersed in nitrogen. The
problem is that you have a working distance then
You need to transfer your sample between different microscopes. This can cause: ice
contaminations, devitrification, grids fragility etc.
There are different approaches for correlative cryo imaging
1. Two-in-one microscope
have no transfer and therefore you minimize contamination.
Easier correlation
Compromised performance (you cannot use the best LM and EM there is)
Example I: LM and EM after each other. You turn
your sample
Sample vitrification cryo LM/FM EM
Example II: image both EM and LM at the same time. When electrons hit the sample,
also photons are emitted from your sample (cathodoluminescence)
2. Tandem microscopes
Flexibility: you can use the start-of-art LM and EM
Can capture dynamics (e.g. fluorescence before and after vitrification)
Combined with super-resolution microscopy
Need transfer
Software compatibility between the systems
Example I: LM on top of your plunger. The advantage of this
is that you can image your sample before and after plunging.
This allows you to see if your point of interest is on the same
place.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller annickkooij. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.24. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77858 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now