Summary
Samenvatting 6: enzymkinetiek deel 1
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Enzymkinetiek deel 1
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6 ENZYMKINETIEK DEEL 1Leerdoelen
o Het begrip induced-fit kunnen uitleggen
o Michaelis-menten enzymkinetiek model kunnen uitleggen
o Experimenten kunnen opzetten om de KM en Vmax te bepalen
o Michaelis-Menten grafiek kunnen tekenen en hieruit de K M en VMAX kunnen afleiden
o Een lineweaver-burk plot kunnen tekenen en hieruit de K M en VMAX kunnen afleiden
Substraten
Enzym-substraat
complex
Actief
centrum
Producten
Substraat-specificiteit: induced fit: Een enzym sluit zich rondom het substraat het substraat past
niet precies in de ‘binding site’ van het vrije enzym
Meten van de reactiensnelheid
o Volg de ontwikkeling van product P
o Bepaal daaruit de reactiensnelheid (= omzetting per tijdseenheid)
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