100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Laboratory Procedure to Analyze The DNA $7.49
Add to cart

Other

Laboratory Procedure to Analyze The DNA

 9 views  0 purchase
  • Course
  • Institution
  • Book

This assignment describes the real practical procedure of DNA extraction, PCR procedure and gel electrophoresis of human DNA.

Preview 3 out of 19  pages

  • January 9, 2021
  • 19
  • 2013/2014
  • Other
  • Unknown
avatar-seller
Table of content

Contents Page number

1. Introduction ………………………………………………………………5

2. Objective ……………………………………………………………....7

3. Materials ……………………………………………………………………………………………………. 7

4. Method
4.1 Subject ………………………………………………………………8
4.2 Blood sampling ………………………………………………………8
4.3 Isolation of DNA from blood sample ………………………………9
4.4 Estimating the concentration of genomic DNA
4.4.1. Preparation of agarose gel ………………………………………10
4.4.2. Quantifying and quality analysis of DNA ……………………....11

5. Result ………………………………………………………………………12

6. Discussion ………………………………………………………………13

7. Appendix ………………………………………………………………16

8. References ………………………………………………………………18




1|Page

, List of figures
Figure 1. DNA structure ……………………………………………………………………..5

Figure 2. Refrigerated centrifuge machine ……………………………………………..6

Figure 3. Venepunture procedure ……………………………………………………………..8

Figure 4. DNA extraction observed in pellet ……………………………………………..9

Figure 5. Extracted DNA washed by 70% ethanol ……………………………………10

Figure 6. DNA in the eppendorf tube after washing …………………………………....10

Figure 7. Agarose gel preparation method …………………………………………....11

Figure 8. Movement of Sample DNA and different DNA markers on the gel visualized by UV
light in quantum S4 ……………………………………………………………………………12




2|Page

, List of tables
Table 1. Different concentration markers of different DNA samples ……………………11

Table 2. Different dyes to indicate different sizes of DNA ……………………………………14




3|Page

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller NJayabalan. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $7.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

53068 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$7.49
  • (0)
Add to cart
Added