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Samenvatting Herhaling: bacteriologie 1, Bijhorend bij medische Bacteriologie 2 . $4.93
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Samenvatting Herhaling: bacteriologie 1, Bijhorend bij medische Bacteriologie 2 .

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In dit word-document haal ik de belangrijkste zaken aan van het vak medische bacteriologie 1. Deze kennis is nodig voor het instuderen van medische bacteriologie 2 in het derde jaar. De zaken die aanbod komen, werden ook door Mevrouw Van Mechelen aangehaald als "te kennen herhaling van het vori...

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  • January 13, 2021
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  • 2020/2021
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Medische bacteriologie 2


Herhaling Bacteriologie 1

1. Bodems
- McConckey = Selectieve bodem voor gram negatieve bacteriën (door galzouten op
de bodem, gram+ kunnen hier niet tegen).


→ niet lactose-vergister = kleurloze
kolonies
→ lactose fermenteerder = paarse
kolonies
→ lactose fermenteerder = paarse
kolonies+achtergrond (doordat de
galzouten in bodem neerslaan) = altijd E.
coli!!

(glucose vergisters zijn mogelijk aanwezig
op deze bodem maar dit zie je niet op
McConkey)


Klebsiella, Enterobacter, E.coli = lactose-fermenteerders
Serratia = trage lactose-fermenteerder (kleurloos tot lichtroze kolonie)
Shigella, Salmonella, Proteus = niet-lactose fermenteerders
Pseudomonas = kleurloze kolonies = niet-lactose fermenteerders
Pseudomonas aeruginosa = blauw/groene kolonies door pyocyanine!!

- BHI = universele bodem, voedingsrijke bodem → hierop groeien bijna alle bacteriën!

, - EMB = Eosine methyleen blauw, selectieve bodem voor gram- (gram+ wordt
afgeremd door eosine methyleenblauw.)
→ lactose-positieve kolonies: roze-paars (Enterobacter cloacae, Klebsiella
pneumoniae)
→ lactose-negatieve kolonies: kleurloos (Salmonella typhi, Shigella sonnei, Proteus
vulgaris)
→ E. coli: donkere, blauwe kolonies met groene metaalglans (goede indicatie voor E.
coli!!) = lactose-positief

Duidelijk E. coli
links!




= lactose fermenteerders!




= niet-lactose fermenteerders!

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